Telomere Length Measurement for Human Hepatocytes

In many organs and tissues, including the liver, telomere shortening in vivo occurs with aging and can be accelerated by various environmental factors. Therefore, measurement of telomere length is expected to provide information to assess the lifespan of cells. For liver cells, the reduction of telomeres with human aging has not been elucidated. Therefore, CD BioSciences provides a systematic telomere length study service for hepatocytes to address liver-related senescence studies.

Telomere Length Variation in the Human Hepatocytes

As we age, the liver undergoes many structural and functional changes. Telomeres are known to shorten with age and this shortening has been associated with an increased incidence of many liver disease processes. It has been found that in healthy aging livers, hepatocytes retain telomere length relative to other intrahepatic lineages. In liver disease states, it was found that hepatocytes had shorter telomeres than other cells. The risk of dying from liver disease is much higher in older patients. In addition, shortened hepatocyte telomeres are associated with a variety of diseases, such as hereditary syndromes, viral hepatitis, NASH, and alcoholic liver disease.

Sample Requirements

We offer solutions for measuring telomere length in hepatocyte samples for researchers. The specific requirements are as follows.

• Liver tissues. Store at -80°C.
• Isolated hepatocytes. Prepare a total hepatocyte suspension and then centrifuge. Then, centrifuge the supernatant further to precipitate the hepatocytes.
• DNA samples. Prepare genomic DNA using Proteinase K and sodium dodecyl sulfate (SDS), followed by repeated phenol-chloroform extraction.

Workflow of Hepatocyte Telomere Length Measurements

We offer telomere length assay services for hepatocytes to measure their relative telomere length and average telomere length, which can help monitor cellular aging and study chronic liver disease.

• Cell types
  Our measurement service is available for individually isolated hepatocytes, Kupffer cells, hepatic stellate cells, and cholangiocytes or a mixture of whole liver tissue cells.

• Telomere length measurement
  Our test analyzes the median, average, and individual telomere lengths of the hepatocytes you are studying to see how hepatocyte telomeres change over time and also to see if hepatocyte telomeres change as you develop certain interventions or drugs. Our technology platform is based on terminal restriction fragment (TRF), quantitative polymerase chain reaction (qPCR), and fluorescence in situ hybridization (FISH) methods that measure telomere length. We specialize in these technologies and are constantly updating them.

• Data analysis

  Data Analysis Services	Details
  Correlation analysis of telomere length with age	We analyze the telomere length data in correlation with the age of the individuals from whom the hepatocytes were obtained. Our services provide insights into the natural changes in telomere length with age, serving as a baseline for further investigation.
  Comparative analysis	We compare the telomere length data between different age groups or cohorts to identify any significant differences in telomere length associated with aging.
  Correlation analysis with aging-related diseases	We also correlate the telomere length data with the presence or progression of aging-related liver diseases, aimed to reveal potential associations between telomere length and disease outcomes.

• Delivery reports
  We are committed to quality and provide test results that are easy to understand and analyze, including tabular and graphical versions.

Why Choose Us?

• Our telomere length test services are affordable, confidential, and convenient.
• We support the study of hepatocyte telomeres in chronic liver disease.

CD BioSciences is an expert in the field of aging and aging-related disease research. If you need telomere length measurement of hepatocyte samples or other samples, please contact us to learn more.

Reference

1. Verma S, et al. Sustained telomere length in hepatocytes and cholangiocytes with increasing age in normal liver. Hepatology, 2012, 56 (4): 1510-1520.