As with other regions of the genome, chromatin composition at the telomere regulates structure, which in turn defines function. Similar to the telomeres of other eukaryotes, mammalian telomeres carry marks of heterochromatin. Alterations in this underlying epigenetic code can have effects on telomere replication and recombination. Research is beginning to emerge that aims to determine the links between changes in telomeric chromatin and their possible roles in aging and disease.
CD BioSciences offers comprehensive services for analyzing the chromatin structure of telomeres. Our expertise encompasses the assessment of these structures to understand their alterations as cells age, utilizing advanced technologies to delineate the intricate relationship between telomere and longevity.
Overview of Our Telomere Chromatin Analysis Services
Structural analysis of telomeric chromatin
We can characterize the organization of telomeric chromatin by negative staining electron microscopy, magnetic tweezers, and single-particle cryo-electron microscopy. Our methodologies provide valuable insights into the structural arrangement and dynamics of telomeric chromatin, contributing to the understanding of aging-related changes at the molecular level.
Chromatin immunoprecipitation (ChIP-seq)
Our ChIP-seq services allow for the genome-wide mapping of telomere-associated proteins and histone modifications, offering a comprehensive view of telomere biology and dynamics within the genome.
Workflow of Telomere Chromatin Analysis in Aging Studies
- Sample collection
We start by collecting biological samples such as blood, tissue, or cultured cells from participants of different age groups. It's important to have samples from both young and old individuals for comparative analysis.
- Telomere chromatin analysis
We examine the architecture and behavior of telomeric chromatin, including the distribution, length, and structure of nucleosomes. Additionally, we conduct ChIP assays to determine the chromatin state of telomeres. This involves cross-linking DNA and associated proteins, shearing the DNA, and immunoprecipitating protein complexes using antibodies against histone modifications or telomere-binding proteins.
- Correlation studies and data analysis
We analyze the ChIP-seq data to understand the epigenetic landscape of telomeres in different age groups and correlate it with telomere chromatin status to comprehend changes in chromatin organization in relation to telomere shortening and aging.
Our Service Features
- Customized analysis
Our approach is not one-size-fits-all. We understand that each aging study has specific requirements, and we tailor our analytical protocols to accommodate these unique needs.
- High-throughput capability
We possess the capability to handle a large volume of samples with accuracy and efficiency. This high-throughput capacity allows us to process numerous samples without compromising on the quality of analysis, providing scalability and flexibility to our clients.
- Comprehensive data interpretation
We are committed to not only producing high-quality data but also to providing a comprehensive interpretation of the findings. Our team of experts is proficient in advanced bioinformatics analysis, enabling us to extract meaningful insights from the complex datasets generated through telomeric chromatin analysis.
CD BioSciences is dedicated to advancing knowledge in the area of telomere chromatin analysis in aging studies by continuously updating and refining technology to reveal the mechanisms of aging. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
