Among the molecular changes associated with aging, alterations in autophagy have become recognized as important features of aging in different species. There is increasing evidence that autophagic activity decreases with age in different tissues in different species. Lysosomal protein hydrolysis is reduced during aging, which impairs autophagy, exacerbates cellular damage, and promotes the development of age-related diseases. At CD BioSciences, we understand the significance of autophagy in the aging process and offer cutting-edge analysis services to investigate the compromise of autophagy during aging.
Overview of Our Compromise of Autophagy Analysis Services
Assessment of autophagic flux during aging
We utilize a combination of approaches, such as fluorescence microscopy, Western blotting, and autophagic substrate degradation assays, to evaluate the dynamics of autophagic flux in the collected samples. Based on these technologies, we can quantify the formation, maturation, and degradation of autophagic structures to assess the efficiency of the autophagy-lysosome pathway during aging.
Investigation of autophagy regulatory pathways during aging
We delve into the intricate regulatory pathways that control autophagy and their dysregulation during aging. Through molecular analysis, we examine the expression and activity of key regulators, such as mTOR (mechanistic target of rapamycin) and AMPK (AMP-activated protein kinase). By elucidating the alterations in these pathways, we gain a deeper understanding of compromised autophagy.
Evaluation of autophagy-related gene expression during aging
We employ techniques such as RNA sequencing or quantitative PCR to analyze the expression levels of key autophagy-related genes and regulatory factors in aging tissues, such as core autophagy machinery components, lysosomal enzymes, and cargo receptors. Based on these, we can identify any alterations in the expression of autophagy-related genes that may contribute to compromised autophagy during aging.
Assessment of autophagy substrate accumulation during aging
Through immunohistochemistry and immunofluorescence techniques, we examine the levels of autophagy substrates in various tissues and cellular models, enabling the quantitative assessment of compromised autophagy during aging processing.
Workflow of Analyzing Compromise of Autophagy During Aging
- Sample acquisition and processing
We obtain tissue samples from aging tissues, cells, or animal models, focusing on tissues that are known to exhibit age-related changes in autophagy, such as the brain, skeletal muscle, or liver. Then, we process the collected samples to extract relevant biomolecules, such as proteins or nucleic acids, for downstream analysis.
- Experimental design and execution
Our experienced scientists collaborate with clients to design experiments tailored to their research objectives. The extracted samples undergo a series of assays to assess autophagy-related parameters.
- Data analysis and interpretation
Once the experimental data is generated, our team employs bioinformatics tools and statistical analyses to unravel the complex datasets. We provide comprehensive reports summarizing the findings, including detailed statistical analyses, visualizations, and interpretations of the results.
| Data Analysis Services |
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| Correlation analysis |
We evaluate potential correlations between autophagic flux measurements and age-related parameters, such as indicators of cellular senescence. We use correlation coefficients to assess the strength and direction of these relationships. |
| Pathway analysis |
We utilize bioinformatic tools and databases to perform pathway enrichment analysis on the genes or proteins involved in autophagy and lysosomal function in clients’ samples. Our services aim to identify age-related dysregulation of specific pathways related to autophagy. |
Our Technology Platform for Studying Compromise of Autophagy
- Next-generation sequencing (NGS)
- Mass spectrometry (MS)
- Live cell imaging, such as fluorescence microscopy and confocal microscopy.
The compromise of autophagy during aging is a complex phenomenon. CD BioSciences offers advanced analysis services to unravel the molecular mechanisms underlying compromised autophagy. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
