All of the aging-associated epigenetic changes participate in the regulation of the aging process and hence contribute to aging-related diseases. Therefore, understanding the epigenetic mechanisms in aging will provide new avenues to develop strategies to delay aging.
Fig. 1 An overview of the aging epigenome. (Wang K, et al., 2022)
Indeed, aging interventions based on manipulating epigenetic mechanisms have led to the alleviation of aging or the extension of the lifespan in animal models. CD BioSciences, a leading company specializing in biological services, offers comprehensive epigenetic analysis services to understand the role of epigenetics in aging.
DNA Modification Analysis Services for Aging Research
CD BioSciences focuses on the analysis of DNA modifications in aging studies, namely cytosine methylation, and hydroxymethylation, through next-generation sequencing methods. The genome is generally hypomethylated during aging. So, we perform whole genome bisulfite sequencing (WGBS), bisulfite oligonucleotide capture sequencing (BOCS), and bisulfite amplicon sequencing (BSAS), to analyze base-specific absolute quantitative at both cytosine-guanine dinucleotide (CG) sites and non-CG sites throughout the genome or within a specific region of interest.
Analysis of Histone Modifications in Aging
CD BioSciences offers high-resolution chromatin immunoprecipitation sequencing (ChIP-seq) services to analyze histone modifications in aging, including methylation, acetylation, phosphorylation, ubiquitination, ADP ribosylation, and others. By mapping the distribution of specific histone marks, we can identify regions of the genome that undergo age-related changes in histone modification patterns.
Analysis of Non-coding RNA Regulation in Aging
Accumulating studies have demonstrated that non-coding RNAs (ncRNAs), which widely regulate gene expression in multiple biological processes at the epigenetic, transcriptional, and post-transcriptional levels, also play a critical role in aging. Our services focus on microRNAs (miRNAs), long non-coding RNAs (lncRNAs), R-loops, and circular RNAs (circRNAs), to study ncRNAs in aging.
Analysis of RNA Modifications in Aging
In addition to investigating ncRNA modifications, CD BioSciences also offers services to analyze modifications in protein-coding RNAs, collectively known as messenger RNAs (mRNAs). By performing high-throughput sequencing of mRNAs and profiling their modifications, we can gain insights into the role of mRNA modifications in the aging process. This analysis further unravels the intricate network of epigenetic modifications involved in aging.
Analysis of Age-Related Chromatin Remodeling
CD BioSciences offers a comprehensive analysis of age-related chromatin remodeling from two main factors, histone-modifying enzymes, and ATP-dependent chromatin-remodeling complexes. These allow us to map open chromatin regions and identify changes in chromatin accessibility between young and old individuals. By examining age-related alterations in chromatin structure, we can identify key regulatory regions and pathways involved in aging.
Epigenetic Aging Clock Development Services
CD BioSciences offers a range of services for the development of epigenetic aging clocks, including clock development, data analysis, validation studies, and others. These services are designed to support our clients in advancing their understanding of aging and facilitating research into private medical and aging interventions.
CD BioSciences offers a range of epigenetic analysis services, including DNA and histone modifications, ncRNA regulation, mRNA modifications, and chromatin remodeling analysis. Through these comprehensive analyses, we can unravel the intricate mechanisms underlying aging. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.
Reference
- Wang K, et al. Epigenetic regulation of aging: implications for interventions of aging and diseases. Signal Transduct Target Ther, 2022, 7 (1): 374.
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