DNA methylation refers to the process of adding a methyl group to the C5 position of a cytosine base in DNA under the action of DNA methyltransferase, thereby producing 5-methylcytosine (5mC). Studies have found that during the aging process and the development of related diseases, the overall DNA methylation level in the genome changes. Therefore, accurate measurement of total 5mC levels can help researchers better understand the role of DNA methylation in aging and related diseases. For quantification of 5mC, the molar amount of the compound is determined primarily using an absolute calibration model to isotopically labeled cytosine 13C15N2, or ratios based on the sum of the molecules of cytosine.
With an advanced technology platform and extensive expertise, CD BioSciences provides customers with global DNA 5mC measurement services to better understand the role of DNA methylation in aging.
Sample Requirements
Our services are available on DNA, cell, and tissue samples from distinct age groups, such as young adults, middle-aged individuals, and elderly subjects, to capture the age-related dynamics of global DNA methylation patterns. Please follow the sample requirements below to prepare the correct sample to give the best chance of a successful assay.
| Sample types |
Minimum concentration |
Minimum amount |
Requirements |
| DNA samples |
> 20 ng/µl |
250 ng |
DNA should be non-degradable and shipped at 4°C. |
| Cell samples |
> 50 ng/µl |
0.2-1 M |
Use enough dry ice for shipping. |
| Tissue samples |
> 50 ng/µl |
5 mg |
Use enough dry ice for shipping. |
Our Global DNA 5mC Measurement Services in Aging Studies
We provide an easy-to-use high-throughput assay to quantify global 5mC levels. Changes in global DNA methylation are observed in cells/tissues of different growth and development states provided by clients in their aging-related research.
Epigenetic aging biomarker analysis
We analyze global DNA methylation patterns associated with aging. Our services involve the assessment of 5mC levels in specific age-related genes or genomic regions, providing valuable insights into the epigenetic mechanisms of aging.
Comparative epigenomic aging studies
We conduct global DNA 5mC measurements on samples from different age groups to identify age-associated epigenetic changes. Our services include a comparative analysis of 5mC patterns in healthy aging versus pathological aging, such as in age-related diseases.
Longitudinal DNA methylation profiling
We provide services for longitudinal monitoring of global DNA methylation patterns in individuals over time. Our services allow for the tracking of epigenetic changes associated with aging, potentially enabling the identification of biomarkers for age-related health outcomes.
Technique Tools for Global DNA 5mC Measurements
We offer methods commonly used to measure global DNA methylation levels, including high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods, enzyme-linked immunosorbent assay (ELISA) assays, spectroscopy, and more.
- HPLC-MS/MS analysis
We utilize HPLC-MS/MS method for quantifying 5mC levels in DNA samples. Our technique allows for the precise separation and quantification of different nucleosides, including 5mC, based on their mass-to-charge ratios.
- Immunological methods
We employ immunological methods, such as ELISA and antibody-based approaches, to detect and quantify 5mC levels. Our methods utilize specific antibodies that recognize and bind to 5mC, allowing for quantification through colorimetric or fluorescent readouts.
Why Choose Us?
- One-stop services. We provide all services from sample preparation to data analysis. Our high-quality technical support can assist with data analysis after submitting the report, at no additional cost. Our specific measurement service process is as follows.
- Innovative technology platforms. We have advanced methylation analysis technology and equipment, which guarantees detection sensitivity readings at the global DNA 5mC levels.
- Fast delivery time. Our detection steps are simplified, the measurement time is greatly reduced, and the report is submitted for you in the fastest cycle according to the number of your samples.
If you want to know more about our global DNA 5mC measurement services or are interested in related research projects, CD BioSciences will do its best to help you. You can contact us by phone or email, our technicians will provide you with satisfactory services.
Reference
- Chowdhury B, et al. Technical advances in global DNA methylation analysis in human cancers. Journal of Biological Engineering, 2017, 11 (1): 10.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
