Analysis of Age-Related Changes of Serum in Animal Models

The effects of aging on serum can include changes in biomarkers, protein levels, and other chemical components. With age, there may be increases in inflammation marker levels, alterations in metabolic substances and hormone levels, changes in protein composition, and a decline in immune function. Analyzing these changes in serum provides valuable insights into the aging process across species and yields important data for studying aging mechanisms. With extensive experience in aging research, CD BioSciences is committed to offering comprehensive serum analysis services specifically designed to evaluate aging in animal models.

Sample Requirements & Serum Preparation

At CD BioSciences, we understand the importance of obtaining high-quality serum samples for reliable aging analysis. Our experts provide clients with detailed instructions on sample collection and preparation. Our specialized serum separation protocols ensure the efficient and consistent isolation of pure serum. We utilize centrifugation techniques to separate the serum from cellular components and clotting factors, preserving the integrity of the samples.

How Do We Analyze Age-Related Changes in Serum?

We employ multifaceted approaches to analyze age-related changes in serum. Our advanced analysis platforms and techniques enable clients to delve deep into the molecular signatures of aging.

Metabolomic and lipidomic analysis services

Our mass spectrometry platforms enable clients to conduct in-depth metabolomic and lipidomic analyses of serum samples. We help clients uncover novel biomarkers and elucidate the intricate metabolic alterations that occur during the aging process by mapping the dynamic changes in the metabolome and lipidome.

Protein profiling services

We employ protein profiling techniques to delve into the serum proteome and uncover age-related protein signatures. We utilize mass spectrometry platforms, such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), to perform in-depth quantitative analysis of the serum proteome. Our services enable clients to measure the levels of a wide range of aging-related proteins, providing valuable insights for the development of anti-aging therapeutics.

Deliverables of Our Serum Analysis Services

Upon completion of a comprehensive serum analysis service, we provide our clients with a detailed report as follows. Depending on the client's research objectives, we also perform comparative analyses to identify age-related changes in the serum profile.

• Quantitative data. We offer detailed tables and graphs showcasing the levels of targeted proteins, metabolites, and other biomolecules in serum samples. Specifically, our services enable quantitative analysis of biomolecules, including but not limited to C-reactive protein (CRP), interleukins (e.g., IL-6, IL-1β), tumor necrosis factor-alpha (TNF-α), insulin-like growth factor 1 (IGF-1), transforming growth factor-beta (TGF-β), vascular endothelial growth factor (VEGF), malondialdehyde (MDA), 8-hydroxy-2'-deoxyguanosine (8-OHdG), total antioxidant capacity (TAC), glutathione (GSH), and glutathione peroxidase (GPx).
• Bioinformatics analysis. Using bioinformatics tools, we interpret aging-related data through pathway analysis, network mapping, and machine learning-based biomarker discovery of aging biomarkers, offering insights into the molecular mechanisms of aging.

At CD BioSciences, we offer comprehensive services to analyze age-related changes of serum in animal models, enabling our clients to unlock the secrets of aging and drive breakthroughs in the field of gerontology. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Yue T, et al. Serum Metabolomic Profiling in Aging Mice Using Liquid Chromatography-Mass Spectrometry. Biomolecules, 2022, 12 (11): 1594.