DNA Damage-Induced Senescence Model Customization Services

Extensive observations suggest that DNA damage accumulates with age and that this may be due to an increase in the production of reactive oxygen species (ROS) and a decline in DNA repair capacity with age. Mutation or disrupted expression of genes that increase DNA damage often results in premature aging.

Fig. 1 DNA damage response is a central mediator in triggering cellular senescence. (Chen JH, et al., 2007)

By developing a model that incorporates DNA damage-induced senescence, our clients can understand the molecular mechanisms driving the aging process. Furthermore, the model can aid in drug development by providing a platform for evaluating drug-target interactions (DTIs) in mitigating the impact of DNA damage on senescence and aging. CD BioSciences is proud to offer our clients a comprehensive range of DNA damage-induced senescence model customization services.

Our Customization Options for Inducing DNA Damage

At CD BioSciences, we understand the importance of tailoring DNA damage-induced senescence models to specific research needs. We offer a range of customization options to meet the diverse requirements of our clients as follows:

Chemically induced senescence

Our team can expose your cells to a variety of approved chemotherapeutic agents, such as doxorubicin, etoposide, or cisplatin, which are known to cause DNA double-strand breaks, adducts, and other types of genotoxic lesions. By fine-tuning the dose and duration of exposure, we can induce a controlled level of DNA damage and trigger a senescence response.

Oxidative stress-induced senescence

We can treat the cells with compounds like hydrogen peroxide (H₂O₂), tert-butyl hydroperoxide (t-BHP), or menadione to generate reactive oxygen species (ROS) and stimulate a DNA damage-driven senescence program.

UV radiation-induced senescence

Exposure to ultraviolet (UV) light, particularly UVB and UVC wavelengths, can cause a range of DNA lesions, including cyclobutane pyrimidine dimers and 6-4 photoproducts. Our irradiation systems allow us to precisely control the dose and duration of UV exposure to induce DNA damage and senescence in your cell models.

Combination treatments

Our experienced scientists also guide clients in selecting the appropriate combination treatment strategy based on research objectives.

Workflow of Customizing DNA Damage-Induced Senescence Models

• Cell culture and treatment. We start by culturing cells of interest under controlled conditions to ensure their optimal growth and viability. Once the cells reach the desired confluency, we treat cells with the selected genotoxic agent(s) at the optimized dose and exposure time.
• Senescence assessment. After the induction of DNA damage, we perform comprehensive senescence assessments to evaluate the efficacy of the treatment. Our services include analysis of senescence-associated markers such as senescence-associated β-galactosidase (SA-β-gal) activity, p16^INK4a and p21^Cip1 expression, heterochromatin foci formation, and telomere attrition.
• Data analysis and reporting. CD BioSciences is committed to delivering high-quality data analysis and comprehensive reports to our customers. We provide detailed reports summarizing the experimental procedures and results, enabling you to make informed decisions and accelerate your research.

CD BioSciences offers comprehensive DNA damage-induced senescence model customization services to meet the diverse needs of researchers in the field. Our customization options, workflow, and featured services enable precise induction of DNA damages, robust senescence assessment, and comprehensive data analysis. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Chen JH, et al. DNA damage, cellular senescence, and organismal aging: causal or correlative? Nucleic Acids Res, 2007, 35 (22): 7417-28.