Preclinical research aims to elucidate the pathophysiological mechanisms of aging-related metabolic diseases, such as type 2 diabetes. Animal models are essential tools in this process, enabling researchers to investigate the mechanisms underlying disease progression and assess the efficacy of potential therapeutics. Among various experimental approaches, the insulin tolerance test (ITT) is commonly used to evaluate insulin sensitivity by measuring blood glucose levels before and after the administration of a controlled dose of insulin.
Fig. 1 Effect of aging upon insulin tolerance.
At CD BioSciences, we have been at the forefront of aging research for decades. We are proud to offer a comprehensive suite of insulin tolerance test services tailored specifically for evaluating age-related changes in animal models.
Overview of Insulin Tolerance Test Services
The insulin tolerance test is a fundamental method for assessing insulin sensitivity. At CD BioSciences, our experts inject insulin into animal models following a fasting period to evaluate their insulin sensitivity. Blood glucose levels are measured at various time points (e.g., 0, 15, 30, 60, 90, 120 minutes) post-insulin injection. By administering a controlled insulin dose and closely monitoring the subsequent changes in blood glucose levels, our team can help clients observe age-associated alterations in insulin sensitivity and glucose homeostasis. We also help clients quantify the rate of glucose disappearance, providing a direct assessment of whole-body insulin action and the potential development of insulin resistance with advancing age.
Workflow of Insulin Tolerance Test Services
- Insulin tolerance test protocol development. For preclinical animal studies, our team of experts works closely with clients to develop customized insulin tolerance test protocols. Key parameters such as insulin dosage, fasting duration, and sampling time points are optimized to generate robust and informative data.
- Insulin tolerance test execution. Using validated animal models, specialized equipment, and rigorous quality control measures, our experienced technicians conduct ITT procedures to minimize variability and ensure data reliability.
- Data analysis and interpretation. We compare the insulin tolerance profiles between aged and young animals. Additionally, we correlate the insulin tolerance test results with other age-related metabolic measurements, such as body weight, body composition, and glucose and insulin levels.
To further enhance the value of our insulin tolerance test services, CD BioSciences also offers the integration of other metabolic assessments, such as body composition analysis and biochemical assays. We assist our clients in gaining a holistic understanding of the complex metabolic alterations associated with the aging process by combining these complementary evaluations. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Buono R, Brandhorst S. Assessing Insulin and Glucose Tolerance in the Aging Mouse. Methods Mol Biol, 2020, 2144: 125-129.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
