Single-Cell Omic Services in Aging Processes

The study of aging at the molecular level is complicated by varying decay characteristics and kinetics—both between and within organs—driven by intrinsic and extracellular factors. Emerging single-cell omics methods allow for molecular and spatial profiling of cells, and probing of regulatory states and cell-fate determination, thus providing promising tools for unraveling the heterogeneous process of aging and making it amenable to intervention.

Fig. 1 Single-cell platforms for studying organismal aging. (He X, et al., 2020)

By leveraging advanced technologies and expertise, CD BioSciences offers cutting-edge single-cell omic services for aging research, including single-cell genomics, transcriptomics, epigenomics, proteomics, and metabolomics services.

Single-Cell Genomic Sequencing for Aging Research

CD BioSciences specializes in single-cell genomic sequencing for aging research, providing valuable insights into aging-related cellular heterogeneity, germline mutations, somatic mutations, and longitudinal profiling. We employ sequencing technologies, such as single-cell whole-genome sequencing and single-nucleus sequencing, to accurately capture genomic information at the single-cell level.

Single-Cell Aging Epigenomic Profiling Services

We offer single-cell epigenomic sequencing services, enabling our clients to investigate DNA methylation patterns, histone modifications, and chromatin accessibility in individual cells. By examining epigenetic profiles across different cell types and aging stages, researchers can identify age-associated epigenetic changes and their functional sequences.

We have developed a variety of single-cell epigenomic sequencing techniques, such as single-cell bisulfite sequencing to measure DNA methylation, single-cell chromatin immunoprecipitation sequencing to measure histone modifications and protein-DNA interactions, and single-cell assay for transposase-accessible chromatin (ATAC) and Hi-C sequencing to measure chromatin accessibility and chromosome conformation.

Single-Cell Transcriptome Profiling for Aging Characterization

We employ single-cell transcriptomic sequencing techniques, such as droplet-based and plate-based single-cell RNA sequencing, to examine gene expression profiles at a single-cell resolution. With our comprehensive transcriptome data analysis services, we can identify senescence-related cellular subpopulations, biomarkers, trajectory analysis, and pseudo-time inference. By characterizing the transcriptomes of different cell types and aging stages, our clients can identify age-related changes in cellular function and identify key genes and pathways associated with aging.

Single-Cell Proteomics Services in Aging Studies

Our single-cell proteomics services allow us to investigate protein profiles in individual cells. By examining protein abundance, localization, and modifications, researchers can gain a deeper understanding of age-related changes in cellular processes and signaling pathways. Our advanced technologies, such as mass cytometry and single-cell western blotting, enable multiplexed protein analysis at the single-cell level.

Single-Cell Metabolomic Profiling of Aging

We utilize a comprehensive range of technologies, such as mass spectrometry and microfluidic platforms, to enable high-throughput metabolite analysis at the single-cell level. Our expertise in data analysis ensures accurate interpretation of metabolomic data. By examining the metabolomes of different cell types and aging stages, researchers can identify age-related metabolic alterations and their impact on cellular function.

Single-Cell Aging Clock Development Services

We offer comprehensive single-cell aging clock development services that allow for the precise measurement and analysis of cellular aging. Our approach provides a more nuanced understanding of aging, as it takes into account the heterogeneity that exists among cells within a population.

Related Supporting Services

• CD BioSciences provides end-to-end solutions for single-cell omic analyses, from sample preparation to data analysis. Our scientists guide researchers through the entire process, ensuring efficient and reliable results.
• We utilize state-of-the-art technologies and platforms for single-cell omic analyses, including advanced sequencing technologies, mass spectrometry, and microfluidics. These technologies enable comprehensive and accurate profiling of genomic, epigenomic, transcriptomic, proteomic, and metabolomic information at the single-cell level.
• We employ advanced computational algorithms and statistical methods to extract meaningful insights from complex datasets, providing comprehensive and interpretable reports of experimental results.

Through our advanced technologies, expertise, and end-to-end solutions, CD BioSciences enables our clients to unravel the intricate molecular changes that occur at the single-cell level during the aging process. If you are interested in our services or have any questions, please feel free to contact us to discuss how we can be involved in your research program.

Reference

1. He X, et al. Single-cell omics in aging: a young and growing field. Nat Metab, 2020, 2 (4): 293-302.