DNA modification services are designed such that either methylation or both methylation and hydroxymethylation can be examined across the entire genome, every gene, or select genes. It also plays a crucial role in gene expression and can provide valuable insights into age-related changes at the molecular level. CD BioSciences focuses on the epigenetic domain of DNA modification intending to understand the epigenetic mechanisms of aging and age-related diseases.
Whole Genome Oxidative/Bisulfite Sequencing for Aging Studies
CD BioSciences provides whole genome oxidative/bisulfite sequencing (WGox/BS) services for the comprehensive analysis of DNA modifications in the entire genome. We modify genomic DNA with bisulfite, transform variants with bisulfite, and then create sequencing libraries. This process involves extensive sequencing. We use it in aging studies, which can help clients determine whether the promoter regions of a specific set of genes change with age.
Reduced Representation Bisulfite Sequencing for Aging Studies
We employ reduced representation bisulfite sequencing (RRBS) as a cost-effective alternative to whole genome sequencing, focusing on a subset of genomic regions enriched for functional elements. By comparing DNA methylation patterns between young and aged individuals, we help our clients unravel age-associated changes in specific genomic regions, shedding light on the epigenetic regulation of aging processes.
Oxidative/Bisulfite Oligonucleotide Capture Sequencing for Aging Studies
We offer oxidative/bisulfite oligonucleotide capture sequencing (Ox/BOCS) services that utilize customized capture probes to enrich regions of interest before subsequent bisulfite treatment and sequencing. CD BioSciences has developed advanced capture probe designs for a variety of species, enabling precise and accurate identification of age-associated DNA modifications. Specifically, we have probe sets for the human, mouse, and rat genomes and can design probes for any annotated genome.
Oxidative/Bisulfite Amplicon Sequencing for Aging Studies
CD BioSciences has optimized oxidative/bisulfite amplicon sequencing (Ox/BSAS) for studying aging-related DNA modifications, enabling our clients to investigate specific gene loci implicated in aging processes. By focusing on candidate genes known to be involved in age-related pathways, our services give insights into how DNA modifications contribute to the aging phenotype.
Comparison of Sequencing Methods
We compare different sequencing methods that analyze the role of DNA modification in aging to help our patrons who are new to DNA modification research. Considering genomic coverage requirements, sample throughput, and resources available, the current range of technologies can meet most needs.
CD BioSciences offers a range of advanced sequencing techniques, including WGox/BS, RRBS, Ox/BOCS, and Ox/BSAS, to facilitate comprehensive investigations into DNA modifications in aging. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
