Epigenetic regulation through DNA methylation (5mC) plays an important role in aging and a variety of aging-related diseases. Oxidative/bisulfite oligonucleotide capture sequencing (Ox/BOCS) allows for the comprehensive analysis of DNA methylation changes associated with aging. These changes may be reflective of age-related diseases, cellular senescence, and other biological processes related to aging. CD BioSciences offers comprehensive Ox/BOCS services to facilitate aging studies and advance our understanding of the aging process.
Overview of Our Oxidative/Bisulfite Oligonucleotide Capture Sequencing Services
CD BioSciences targets bisulfite sequencing to specific genomic regions by using oligonucleotide probes designed for sequence capture, retaining the benefits of bisulfite sequencing while focusing sequencing reads on regions of interest. By using this approach, we reduce the amount of sequence required per sample, analyze more samples, and provide base- and strand-specific absolute quantification of CG and non-CG methylation levels.
Epigenetic profiling
With Ox/BOCS, we can analyze DNA methylation patterns in the genome of aging individuals. This can help identify specific methylation changes associated with aging and age-related diseases.
Biomarker discovery
We compare DNA methylation profiles between younger and older individuals, to discover epigenetic biomarkers that can potentially be used for age-related disease prediction, diagnosis, or monitoring.
Longitudinal studies
We utilize Ox/BOCS to track changes in DNA methylation patterns over time within individuals. Longitudinal studies can provide insights into the dynamic epigenetic changes associated with the aging process.
Treatment response monitoring
We evaluate the effects of potential anti-aging interventions or therapies on DNA methylation patterns using Ox/BOCS. This approach can be used for a variety of anti-aging strategies that we offer, such as drug discovery, cell reprogramming, and nutraceutical development.
Guidelines for Sample Preparation
CD BioSciences provides detailed guidelines for sample collection, preservation, and processing to our clients. It is essential to collect an adequate number of cells or tissues from individuals of different age groups (young, middle-aged, and old) to capture the diversity of aging-related DNA methylation patterns. In addition, depending on the purpose of the experiment, a healthy older adult population may also be used as a control to study people with age-related diseases.
In addition to collecting samples from humans, we also support the sample collection from aging animal models, such as rhesus, monkeys, common marmosets, mice, rats, fish, etc.
Our Workflow of Oxidative/Bisulfite Oligonucleotide Capture Sequencing Services
Fig. 1 General principles of BOCS.
CD BioSciences offers one-stop services for Ox/BOCS in aging research, including but not limited to probe design, qualification of standards, generation of library through blunt end repair, A-tailing, and adapter ligation, bisulfite conversion, enrichment for regions of interest using hybridization to oligonucleotide probes, next-generation sequencing, and bioinformatics.
We begin with the design of probe sets for the human, mouse, and rat genomes and can design probes for any annotated genome. Once the sequencing results are obtained, our powerful bioinformatics can compare the methylation levels between different age groups or conditions of interest. This analysis can identify differentially methylated regions (DMRs) associated with aging.
Through our comprehensive Ox/BOCS services, CD BioSciences enables our clients to gain valuable insights into the DNA methylation patterns associated with aging. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
