5-Hydroxymethylcytosine (5hmC) is an epigenetic marker essential for gene regulation, and its alterations have been linked with various age-related diseases, such as neurodegenerative disorders and cancer. In contrast to 5-methylcytosine (5mC), the content of 5hmC is much lower in adult mammals. The distribution of 5hmC is variable, tissue-specific, and non-random, which directly influences the equilibrium of DNA methylation in normal physiological activities. By quantifying global DNA 5hmC levels, researchers can gain insights into the epigenetic changes associated with aging and age-related diseases. CD BioSciences offers global DNA 5hmC measurement services for aging studies, providing valuable insights into epigenetic modifications that occur with age.
Sample Requirements
Global DNA 5hmC measurement requires high-quality samples. To investigate aging, our services cover a wide range of age groups, including young, middle-aged, and older adults, depending on the specific research goal. Our global DNA 5hmc measurement services accommodate diverse sample types, including but not limited to:
|
Sample types |
Minimum input quantity |
| DNA |
250 ng |
| Cells |
0.2-1 M |
| Tissues |
5 mg |
Our Global DNA 5hmC Measurement Services in Aging Studies
Global DNA 5hmC profiling
Our services provide a comprehensive analysis of 5hmC levels throughout the genome. By examining the distribution and abundance of 5hmC marks, our clients can gain insights into the epigenetic changes associated with aging and age-related diseases.
Aging-related epigenetic analysis
Our services compare 5hmC profiles across different age groups and monitor 5hmC levels with the same objectives over an extended period. By examining how 5hmC patterns change with age, our clients can identify age-related epigenetic signatures and potential biomarkers of aging. By repeatedly measuring 5hmC at multiple time points, our clients can assess how epigenetic patterns change over time and gain a deeper understanding of the dynamics of aging.
Biomarker discovery
Our services help clients identify specific genomic regions or combinations of 5hmC marks that show strong associations with chronological age. These biomarkers can be further validated and explored for their utility in predicting biological age or assessing age-related health outcomes.
Our Global DNA 5hmC Measurement Methods
CD BioSciences employs multifaceted techniques for global DNA 5hmC measurement, offering highly sensitive and specific assays to measure 5hmC levels in diverse sample types quantitatively. Our methods include next-generation sequencing methods, LC-MS-based approaches, ELISA-based assays, and colorimetric assays. Our methods enable the reliable quantification of global DNA 5hmC, empowering clients to make robust interpretations of age-related epigenetic modifications.
Deliverables of Global DNA 5hmC Measurements in Aging Studies
Our commitment to delivering comprehensive and actionable results underpins the core of global DNA 5hmC measurement services in aging studies.
- Detailed 5hmC analysis reports. We provide meticulously curated reports encompassing detailed 5hmC profiles, highlighting age-related variations in 5hmC levels across genomic regions of interest, fostering a nuanced understanding of epigenetic alterations in the aging process.
- Age-associated differentially hydroxymethylated regions (aDhMRs). We offer reports of specific genomic regions that show differential 5hmC levels between different age groups.
At CD BioSciences, our global DNA 5hmC measurement services are fundamental to unraveling the complex mechanisms of aging. By offering unparalleled reliability and expertise, we are committed to advancing the frontiers of aging research through our innovative global DNA 5hmC measurement services. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
