Targeted bisulfite sequencing is a powerful tool that allows for the precise analysis of DNA methylation patterns at single-nucleotide resolution. This method involves the treatment of DNA with sodium bisulfite, which converts unmethylated cytosines to uracil while leaving methylated cytosines unaltered. Subsequent amplification and high-throughput sequencing of the treated DNA enable the precise profiling of DNA methylation at targeted genomic regions. This technique is essential for investigating the epigenetic changes associated with aging and age-related diseases.
By leveraging cutting-edge technologies and expertise, CD BioSciences offers advanced targeted bisulfite sequencing services, catering to the specialized needs of aging research studies.
Sample Requirements
Our experts recommend that clients provide high-purity genomic DNA extracted from the desired tissue or cell types relevant to their aging studies. We ensure that the sample requirements are met to guarantee optimal sequencing outcomes and data reliability.
Sample types |
Minimum input quantity |
DNA |
500 ng |
Cells |
0.2-1 M |
Tissues |
5 mg |
Workflow of Targeted Bisulfite Sequencing
CD BioSciences employs meticulously designed workflows for targeted bisulfite sequencing, incorporating advanced methodologies and quality control measures. Our comprehensive workflow ensures the detailed profiling of DNA methylation, providing valuable insights into the epigenetic mechanisms underlying aging processes.
- Treatment of genomic DNA. We treat genomic DNA with sodium bisulfite, converting unmethylated cytosines to uracil while preserving methylated cytosines.
- Targeted amplification. We use specific primers to amplify the regions of interest, enabling the selective enrichment of methylated and unmethylated cytosines.
- High-throughput sequencing. We utilize next-generation sequencing platforms for high-throughput sequencing of amplified DNA fragments to generate high-resolution DNA methylation profiles.
- Bioinformatics analysis. Our bioinformatics experts employ sophisticated algorithms and robust analytical pipelines to interpret the sequencing data, elucidating the methylation patterns associated with aging-related genomic regions.
Applications of Targeted Bisulfite Sequencing in Aging Research
Applications of targeted bisulfite sequencing in aging research encompass various aspects of the epigenetic investigation, offering profound insights into the molecular mechanisms underlying age-related processes and diseases.
Examination of age-dependent DNA methylation patterns
Our targeted bisulfite sequencing services allow clients to investigate DNA methylation patterns at specific genomic regions of interest, such as CpG islands, gene promoters, or regulatory regions. By comparing DNA methylation profiles between individuals of different ages, our clients can identify specific CpG sites or regions that show consistent changes in DNA methylation with increasing age.
Development of epigenetic clocks
Our targeted bisulfite sequencing services can be used to assess DNA methylation patterns at specific CpG sites that are known to change with age. By analyzing these patterns, our clients can develop epigenetic clocks, which are mathematical models that estimate an individual's biological age based on their methylation profile.
Biomarker discovery
Our targeted bisulfite sequencing services can help identify DNA methylation biomarkers associated with aging and aging-related diseases. By comparing methylation profiles between young and old individuals or healthy and diseased individuals, our clients can identify differentially methylated regions (DMRs) that are specific to aging or aging-related pathologies. These DMRs can serve as potential biomarkers for diagnostic or prognostic purposes.
CD BioSciences is committed to providing unparalleled targeted bisulfite sequencing services for aging studies, facilitating groundbreaking discoveries in the field of aging research. Our comprehensive approach, combined with advanced technologies and expertise, offers clients the opportunity to gain profound insights into the epigenetic landscape of aging. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.