Mitochondria play a central role in energy metabolism. Recently, gerontological studies have identified various molecular pathways involved in aging and highlighted mitochondria as a key regulator of longevity. At CD BioSciences, we specialize in the analysis of mitochondrial energy metabolism disorders associated with aging.
Overview of Mitochondrial Energy Metabolism Disorder Analysis Services
Assessment of mitochondrial function in aging
We employ various approaches to evaluate mitochondrial function, including measurements of mitochondrial membrane potential, ATP production, oxygen consumption rate (OCR), and reactive oxygen species (ROS) production. By quantifying these parameters, we gain insights into age-related changes in mitochondrial function and their energy-generating capacity during the aging process.
Specifically, we use fluorescence-based assays such as JC-1 or TMRM for quantitative assessment of mitochondrial membrane potential. We analyze the ATP levels in aging cells or tissues to understand the impact of mitochondrial energy metabolism disorders on cellular bioenergetics. In addition, we also quantify ROS production in conjunction with mitochondrial energy metabolism disorders to understand the associated oxidative stress and its implications for aging.
Analysis of metabolic pathway in aging
Metabolic profiling and pathway analysis are crucial for understanding the alterations in energy metabolism associated with aging. We employ state-of-the-art metabolomics technologies, such as mass spectrometry and nuclear magnetic resonance (NMR), to comprehensively analyze the metabolic profile of aging samples. Therefore, we can identify changes in key metabolites and pathways involved in mitochondrial energy metabolism disorders during the aging process.
Investigation of cellular responses in aging
We investigate the activation of key signaling pathways, such as AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), which regulate mitochondrial biogenesis and function. Our analysis services shed light on the cellular mechanisms involved in compensating for mitochondrial energy metabolism disorders during the aging process.
Workflow of Analyzing Mitochondrial Energy Metabolism Disorder During Aging
- Experimental design
We design experiments to investigate mitochondrial energy metabolism during aging, involving choosing appropriate model systems (e.g., cells, animal models, human samples), selecting specific assays for mitochondrial function assessment, and determining experimental endpoints.
- Sample collection and mitochondrial isolation
We obtain biological samples from aging individuals or relevant model organisms for analysis. We isolate mitochondria from the collected samples using established protocols to study their bioenergetic properties and evaluate mitochondrial function.
- Biochemical and molecular analysis
We perform biochemical analyses to measure key parameters such as mitochondrial respiratory chain complex activities, ATP production, reactive oxygen species (ROS) levels, and mitochondrial membrane potential. What's more, we conduct molecular analyses to assess mitochondrial DNA (mtDNA) integrity, gene expression profiles of mitochondrial proteins, and levels of mitochondrial biogenesis regulators.
- Data analysis and interpretation
We integrated data obtained from biochemical and molecular analyses to interpret the results in terms of disturbed mitochondrial energy metabolism during aging.
Applications of Mitochondrial Energy Metabolism Disorder Analysis in Aging Studies
Identification of biomarkers
Through our analysis services, our clients identify and validate biomarkers that can be used for early detection, prognosis, and monitoring of mitochondrial energy metabolism disorders during aging.
Assessment of mitochondrial healthspan
Mitochondrial healthspan refers to the functional capacity of mitochondria throughout the aging process. By analyzing mitochondrial energy metabolism disorders, our clients gain insights into the factors that influence mitochondrial healthspan.
CD BioSciences provides a comprehensive range of analysis services employing various techniques to unravel the enigmas of mitochondrial energy metabolism disorders during the aging process. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
