Nuclear DNA (nDNA) is subjected to a constant barrage of damage, mostly hydrolysis, oxidation, and alkylation. Evidence is presented that damage to nDNA is a direct cause of aging in addition to the effects of nDNA damage on cancer, apoptosis, and cellular senescence.
Fig. 1 DNA damage is the driver of aging. (Schumacher B, et al., 2021)
Many studies show significant nDNA damage with age, associated with declining nDNA repair. CD BioSciences, a company specializing in aging research and analysis, offers a comprehensive analysis of nuclear DNA damage caused by aging.
Types of Aging-Related Nuclear DNA Damage We Serve for Analysis
The aging process is associated with the accumulation of various types of nuclear DNA damage. We provide analysis services for several types of DNA damage associated with aging, including but not limited to the following:
- CD BioSciences assesses DNA strand breaks and evaluates the extent of DNA damage in the comet assay. Based on this, we assess the genomic destabilization and damage to various cellular processes resulting from these breaks. By quantifying the extent of DNA damage in aging samples, our clients can gain insights into the impact of aging on nuclear DNA integrity.
- We offer specialized analysis services to quantify and characterize 8-oxo guanine formation in aging samples. We employ immunofluorescence and mass spectrometry-based techniques to detect and quantify 8-oxo guanine levels, providing valuable information on oxidative DNA damage during aging.
Sample Requirements for Analyzing Aging-Related Nuclear DNA Damage
- Tissue samples
We obtain tissue samples from individuals across different age groups, preferably from the same tissue or organ to minimize variability. Common tissue types studied in aging research include blood, skin, liver, and brain.
- Fresh vs. frozen samples
We recommend using freshly collected samples for DNA damage analysis to minimize the potential for degradation. However, if fresh collection is not possible, rapid freezing of samples at ultra-low temperatures (-80°C) will also maintain DNA integrity for subsequent analysis.
Workflow for Nuclear DNA Damage Analysis Services
- DNA extraction
We extract the nuclear DNA from the collected samples using established laboratory protocols. We ensure the DNA extraction process is robust and optimized to obtain high-quality DNA.
- DNA damage assessment
We use several techniques to analyze DNA damage. The table below shows the methods we commonly use.
| Detection Methods |
Details |
| Single-cell gel electrophoresis (comet assay) |
We use this assay to measure DNA strand breaks and base solubility sites. Cells are embedded in agarose gel, subjected to electrophoresis, and stained with a fluorescent dye. Damaged DNA migrates away from the nucleus, forming a "comet tail" that can be quantified. |
| Immunofluorescence |
We label and visualize damaged DNA in cells with antibodies specific for specific DNA damage, such as 8-oxo guanine (a marker of oxidative damage.) We can also provide quantification by fluorescence microscopy or flow cytometry. |
- Data analysis and interpretation
We employ advanced bioinformatics tools and statistical methods to analyze and interpret the data obtained from nuclear DNA damage analysis. Correlations between DNA damage levels and age can be identified, enabling the identification of potential biomarkers or pathways associated with the aging process.
Focusing on key aspects such as DNA strand breaks and the formation of 8-oxo guanine, CD BioSciences provides researchers with valuable insights into the molecular alterations occurring during aging. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Schumacher B, et al. The central role of DNA damage in the aging process. Nature, 2021, 592 (7856): 695-703.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
