Whole Exome Sequencing Services in Aging

The areas of the genome that encode functional proteins are called exons. Whole exome sequencing (WES) is a next-generation sequencing (NGS) technique that allows for the efficient and cost-effective sequencing of the exome, which comprises the protein-coding regions of the genome. It provides valuable information about variations and mutations, which can be potential contributors to the aging process or age-related diseases. At CD BioSciences, our bioinformatics team provides one-stop technical services for your exome sequencing project.

Overview of Our Whole Exome Sequencing Services

CD BioSciences has developed a state-of-the-art WES platform that combines advanced sequencing technologies with robust bioinformatics analysis pipelines. Our comprehensive approach encompasses targeted capture, library preparation, sequencing, and data analysis, enabling us to provide our clients with accurate and detailed information on the genetic variations present in the exome.

Identification of genetic variants in aging

Utilizing WES, we can identify rare or common genetic variants that are associated with aging-related traits, including age-related diseases, longevity, or age-associated phenotypes.

Discovery of novel genes in aging

By examining the entire exome, we offer the service of discovering unknown genes that may be involved in the aging process.

Guidelines for Sample Preparation

• Sample types
  Our sequencing services support a variety of sample types, including blood (e.g., peripheral blood mononuclear cells or whole blood), tissue samples (e.g., skin or muscle biopsies), and saliva samples. The specific sample type depends on the specific goals of our client's research.
• DNA quantity

Our Workflow of Whole Exome Sequencing Services

We offer a range of services for whole exome sequencing in aging research. Our workflow follows a standardized protocol that ensures reproducibility and data quality.

Fig. 1 Technical routes for whole exome sequencing.

• Consultation and study design
  Our team of experts works closely with our clients to understand their research on aging issues and design studies that meet specific requirements.
• Sample collection and DNA extraction
  We collect biological samples such as blood, skin, or tissues from the longevity cohort. Or, we collect samples from people who have experienced age-related diseases. Our featured sample collection work is on aging animal models, such as primate models and rodent models. We provide detailed sample collection guidelines and assist clients in obtaining high-quality DNA.
• Library preparation and targeted capture
  We utilize state-of-the-art library preparation methods to enrich the exonic regions of interest. In the enrichment strategy, we can incorporate probes that target regions associated with aging or age-related diseases.
• Next-generation sequencing
  We sequence the enriched libraries using high-throughput NGS platforms, generating millions of DNA fragments that represent the exome.
• Bioinformatics analysis
  Our team of bioinformatics experts employs cutting-edge algorithms and software tools to analyze the sequencing data, identify genetic variations (single nucleotide variants or SNVs, insertions, deletions, and structural variants), and annotate their potential functional significance related to aging.

CD BioSciences follows rigorous quality control measures throughout the entire workflow of WES to ensure the accuracy and reproducibility of the sequencing results. If you are interested in our services, please feel free to contact us or make an online inquiry.