Research on the molecular mechanisms of cellular aging has mainly focused on protein-coding genes. However, accumulating studies have demonstrated that non-coding RNAs (ncRNAs), which widely regulate gene expression in multiple biological processes at the epigenetic, transcriptional, and post-transcriptional levels, also play a critical role in aging. At CD BioSciences, we offer a comprehensive suite of ncRNA regulation analysis services to unravel the intricate mechanisms underlying aging.
Fig. 1 The mechanism of ncRNA regulation during aging. (Wang K, et al, 2022)
Overview of Our Non-Coding RNA Regulation Analysis Services
Analysis of microRNAs (miRNAs) in aging
MiRNAs are small (19-22 nucleotides), non-coding, and single-stranded RNAs that have become an emerging aging research interest over the last few years. CD BioSciences offers advanced miRNA analysis services using a microarray that can identify up or down-regulated expression of miRNAs during aging.
By identifying dysregulated miRNAs in aging, we can gain insights into the molecular mechanisms underlying age-related changes and potentially identify novel therapeutic targets. Our services can support the use not only in animal models such as Caenorhabditis elegans and Drosophila melanogaster but also in human biological samples.
Analysis of long non-coding RNAs (lncRNAs) in aging
LncRNAs are non-protein-coding RNAs longer than 200 nucleotides. CD BioSciences utilizes sophisticated techniques, such as RNA sequencing and computational analysis, to identify differentially expressed lncRNAs in aging.
We also identify the lncRNAs implicated in diseases arising from age-driven degeneration or dysfunction in some representative organs and systems (brains, liver, muscle, cardiovascular system, bone pancreatic islets, and immune system). By integrating lncRNA expression data with other omics data, we can unravel the functional roles of lncRNAs in aging and highlight their potential as biomarkers or therapeutic targets.
Analysis of circular RNAs (circRNAs) in aging
CircRNAs are a unique class of ncRNAs that form covalently closed continuous loops without linear ends. We provide analyses of the role of circRNAs in the modulation of aging and aging-related diseases such as cardiovascular disease, diabetes, and neurodegenerative diseases. Since circRNA is relatively stable, we are also developing potential diagnostic biomarkers for aging using global circRNA levels.
Our Non-Coding RNA Regulation Analysis Techniques
Based on our advanced platform, we comprehensively analyze the role of non-coding RNA in aging. Currently, the main methods used for non-coding RNA research include transcriptome sequencing, Northern blotting, real-time fluorescence quantitative PCR technology, fluorescence in situ hybridization (FISH), RNA interference, RNA binding protein immunoprecipitation, etc. Among them, the fluorescence quantitative PCR method, as the gold standard for the detection of non-coding RNA expression, is more widely used.
CD BioSciences, with its expertise in the analysis of microRNAs, long non-coding RNAs, R-loops, and circular RNAs, is dedicated to unraveling the intricate molecular mechanisms underlying the aging process. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Wang K, et al. Epigenetic regulation of aging: implications for interventions of aging and diseases. Sig Transduct Target Ther, 2022, 7: 347.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
