Unlike nuclear DNA, mitochondrial DNA (mtDNA) contains only exons and lacks histone protection and an effective gene repair system. These features make mtDNA more prone to mutation than nuclear DNA. Mutations in mtDNA cause a variety of human mitochondrial diseases and are also strongly implicated in age-related diseases and aging.
Fig. 1 Schematic representation of mammalian mtDNA. (Park CB and Larsson NG, 2011)
CD BioSciences offers comprehensive mitochondrial DNA mutation analysis and intrinsic mechanism analysis services. Based on our expertise, our high-quality results contribute to a deeper understanding of the aging process.
Our Mitochondrial DNA Mutation Analysis Services
Whole mitochondrial genome sequencing
We sequence the entire mitochondrial genome to identify age-related mutations, including point mutations, deletions, and insertions. So, we provide details of mitochondrial DNA (mtDNA) mutations to identify patterns associated with aging.
Deletion/duplication analysis
Our company offers targeted analysis for common mtDNA deletions and duplications associated with aging. We employ techniques such as long-range PCR or digital PCR to detect and quantify the levels of these structural variants in mitochondrial DNA.
Heteroplasmy analysis
We quantify heteroplasmic mutations, where both mutant and wild-type mtDNA coexist within a cell, using next-generation sequencing or quantitative PCR. Thus, we reveal the dynamics of mtDNA mutation accumulation during aging.
Mitochondrial DNA repair pathway analysis
We assess the expression levels or activity of proteins involved in mtDNA repair pathways, such as base excision repair, mismatch repair, and recombinational repair. Based on this, we provide insights into the efficacy of mtDNA repair mechanisms during aging.
Analysis of the Mechanisms Underlying Mitochondrial Mutations During Senescence
Analysis of mitochondrial DNA mutations by oxidative stress
Our company is helping clients explore the relationship between mitochondrial mutations, oxidative stress, mitochondrial dysfunction, and aging. We analyze oxidative stress-induced release of mtDNA into the cytoplasm and extracellular space with implications for senescence. In particular, we provide an analysis of the pathway by which mtDNA released into the cytoplasm binds to cGAS and activates STING, thereby participating in cellular senescence. In addition, we focus on the release of mtDNA into the extracellular compartment as a signal for mitochondrial communication in relation to senescence-related diseases.
Analysis of metabolic on mitochondrial mutations
We offer an analysis of how organismal energy and metabolism affect mitochondrial mutations, which can shed light on the role of mitochondria in the aging process. We examine how metabolic signaling pathways, including AMP-activated protein kinase (AMPK) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), modulate mitochondrial biogenesis in response to mutations associated with aging.
Analysis of cellular signaling on mitochondrial mutations
We investigate how cellular signaling, such as those mediated by the AMP-activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR), influence mitochondrial mutations in aging. In addition, we are equally concerned with the effects of apoptotic and inflammatory signaling on mitochondrial mutations.
Why Choose Us
- We offer one-stop services for mitochondrial DNA mutation analysis and its intrinsic mechanistic analysis. We offer tailor-made methods to meet the specific needs of our clients.
- We utilize cutting-edge technologies such as next-generation sequencing and advanced bioinformatics tools to provide accurate and reliable analysis results of mtDNA variations.
- We provide detailed analytical reports and data, and do our best to give original aging-related perspectives.
CD BioSciences has developed unparalleled expertise in mitochondrial DNA variation analysis. Our team of highly skilled specialists is well-versed in the intricacies of mtDNA analysis and possesses in-depth knowledge of the molecular mechanisms underlying senescence. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Park CB, Larsson NG. Mitochondrial DNA mutations in disease and aging. J Cell Biol. 2011, 193 (5): 809-18.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
