More than 170 types of RNA modifications have been discovered thus far that regulate gene expression at the epitranscriptomic level. Accumulating research shows the essential roles of these post-transcriptional regulatory mechanisms in the cellular senescence process, one of the critical causes of aging and aging-related diseases. CD BioSciences offers cutting-edge technologies and expertise to investigate various RNA modifications associated with aging.
Fig. 1 RNA modifications involved in senescence. (Wang K, et al, 2022)
Overview of Our RNA Modification Analysis Services
Analysis of N6 methylation of adenosine (m6A) modification in aging
CD BioSciences provides analysis services of the role of m6A modifications in the aging process and age-related diseases, including cell senescence, autophagy, inflammation, oxidative stress, DNA damage, tumors, neurodegenerative diseases, diabetes, and cardiovascular diseases (CVDs).
Our services include m6A-specific antibody-based immunoprecipitation (MeRIP) followed by high-throughput sequencing (MeRIP-seq). we can offer the entire workflow, from sample preparation to data analysis, to generate m6A modification profiles in aging-related samples. We also focus on analyzing this dynamic, reversible m6A modification process with three enzymes, RNA methyltransferases, RNA demethylases, and m6A-binding proteins.
Analysis of 5-methylcytosine (m5C) modification in aging
CD BioSciences provides specialized services for analyzing m5C modification in RNA samples derived from aged individuals. Our advanced techniques, such as bisulfite sequencing, allow us to accurately detect and quantify m5C modification sites with high sensitivity. Through comparative analysis between young and aged samples, we can identify age-related changes in m5C modification patterns and elucidate their potential role in aging-related mechanisms.
Analysis of adenosine to inosine RNA editing (A-to-I editing) in aging
We offer a comprehensive analysis of A-to-I RNA editing events in aging-related samples, especially for aging-related neurological disorders. Our cutting-edge technologies, including RNA-seq and bioinformatics analysis, enable us to identify and quantify A-to-I editing at the nucleotide level. In some cases, we have also assisted in the study of A-to-I editing by using deaminases such as ADARs (Adenosine Deaminases Acting on RNA), which deaminate adenosine to inosine, and then analyzing the modified RNA by sequencing methods.
Our RNA Modifications Analysis Techniques
We have developed RNA modification detection and sequencing methods, such as RNA immunoprecipitation (RIP), m6A individual-nucleotide-resolution cross-linking and immunoprecipitation (miCLIP), CLIP, liquid chromatography-tandem mass spectrometry (LC/MS-MS), and sequencing to discover new modifications and map them to different species of RNA within any aging cell type or model organism.
CD BioSciences offers advanced RNA modification analysis services to unravel the molecular mechanisms of aging, including analysis of m6A modifications, m5C modifications, and A to I editing in aging-related samples. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Wang K, et al. Epigenetic regulation of aging: implications for interventions of aging and diseases. Sig Transduct Target Ther, 2022, 7: 347.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
