Traditional genomic sequencing provides an average of the genetic material within a large population of cells, but single-cell sequencing allows researchers to study the unique genetic variations present in individual cells. Single-cell genomic sequencing has emerged as a powerful tool for aging research, enabling scientists to investigate the genetic and molecular changes that occur at the individual cell level during the aging process. At CD BioSciences, we offer state-of-the-art single-cell genomic sequencing services specifically tailored for aging research.
Overview of Our Single-Cell Genomic Sequencing Services
Cellular heterogeneity analysis in aging
Aging is a complex process involving cellular heterogeneity, where different cells within the same tissue may age at different rates or exhibit distinct molecular profiles. We employ single-cell genomics to characterize this heterogeneity by profiling individual cells and identifying age-related changes.
Analysis of germline and somatic mutations in aging
Our company focuses on identifying DNA mutations in germ cells (sperm cells and oocytes) and somatic cells through the usage of single-cell genomic sequencing. This enables our clients to gain a better understanding of the genetic factors of healthy aging and age-related diseases. In the case of somatic mutations, we can detect uncommon and distinct single nucleotide variations (SNVs) and copy-number variations (CNVs) during the process of aging.
Longitudinal profiling in aging
We perform single-cell sequencing on samples collected at different time points from the same individuals to study the dynamics of aging at the single-cell level. We use this longitudinal approach to reveal how gene expression or cellular composition changes over time, providing a comprehensive understanding of the aging process and potential interventions to slow down or reverse aging.
Technology Platforms for Single-Cell Genome Sequencing
Our single-cell DNA sequencing (scDNA-seq) platform allows the analysis of the genomic DNA content of individual cells, uncovering genomic variations and somatic mutations at a single-cell resolution. If requested, we specialize in combining DNA sequencing information with other assays, such as genomic stability and chromatin accessibility assays, to provide deeper insights into aging mechanistic studies.
Our Workflow for Single-Cell Genome Sequencing
- Sample preparation
We guide sample collection, cell dissociation, and preservation techniques to maintain cell viability and integrity. In aging research, biological samples are usually collected from people or animal models of different age groups, longevity groups, and aging-related diseases.
- Single-cell isolation and library preparation
We employ single-cell isolation technologies such as microfluidics-based platforms or fluorescence-activated cell sorting (FACS), to obtain individual cells for downstream sequencing. Following single-cell isolation, we perform library preparation, including reverse transcription, amplification, and library construction, tailored to the specific sequencing platform.
- Sequencing
We leverage cutting-edge sequencing technologies to perform high-throughput sequencing of the libraries generated from individual cells. Our sequencing platforms ensure deep coverage and high-resolution data, enabling comprehensive analysis of the genomic profiles of individual cells.
- Data analysis
Our team of bioinformatics experts analyzes sequencing data generated from single cells. We perform quality control, data normalization, and statistical analysis to identify age-related changes in gene expression, genomic variations, and epigenetic modifications. We provide comprehensive reports and visualizations, enabling researchers to gain valuable insights into the molecular mechanisms of aging.
At CD BioSciences, our team of scientists and bioinformatics experts possesses extensive knowledge and expertise in single-cell genomics and aging research. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
