Analysis of Histone Modifications in Aging

Aging has been associated with structural changes in chromatin. At the molecular level, multiple histone modifications with established epigenetic mechanisms have been connected to the regulation of lifespan. Post-translational modifications of histones can activate or silence gene expression and regulate the aging process. At CD BioSciences, we specialize in providing cutting-edge histone modification analysis services, which play a crucial role in regulating gene expression and chromatin structure.

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Fig. 1 Redistribution of histone modifications during aging. (Maleszewska M, et al, 2016)

Overview of Our Histone Modification Analysis Services

CD BioSciences supports the analysis of the types of histone modifications including methylation, acetylation, phosphorylation, ubiquitination, and ADP-ribosylation. We analyze the imbalance between repressive and activating histone modifications, which alter the gene expression program and thus promote transcriptomic changes associated with aging.

Analysis of histone methylation in aging

We offer an analysis of the effects of histone methylation on lifespan. The theoretical basis for our services is that methylation can occur on different lysine and arginine residues of histone proteins, leading to either activation or repression of gene expression, thus regulating the lifespan of a species. Our services can be analyzed on a wide range of species, such as Drosophila melanogaster, Caenorhabditis elegans, Saccharomyces cerevisiae, mice, or human biological samples.

We can accurately quantify the levels of specific histone methylation marks in aging samples. By comparing methylation patterns in young and aged samples, we can identify potential changes that occur during aging. We can also determine the effect that different histones have on lifespan by deleting histone methyltransferases, and demethylases.

Analysis of histone acetylation in aging

Our specialized analysis services allow us to examine the levels of histone acetylation in biosamples at different ages. We focus on the role of histone acetyltransferases (HAT) and histone deacetylases (HDAC) in longevity, responsible for the addition and removal of acetyl groups, respectively. These findings contribute to our understanding of the dynamic regulation of gene expression during aging and provide potential targets for therapeutic interventions.

Analysis of histone phosphorylation and ubiquitination in aging

In addition to histone methylation and acetylation, histone phosphorylation and ubiquitination have also been shown to be associated with aging, and in some cases through crosstalk with other histone marks.

By quantifying the levels of histone phosphorylation and ubiquitination, we can identify specific histone marks that change during aging. By analyzing histone phosphorylation and ubiquitination patterns, we can provide insight into the regulation of gene expression and chromatin dynamics during aging.

Our Candidate Library of Histone Markers Involved in Aging and Lifespan Regulation

CD BioSciences is establishing a candidate library of histone marks involved in aging. Future investigation of new aging-related histone marks, as well as the integration of epigenetic data with other areas of cellular activity, will be important to unravel the systems biology mechanisms of aging.

At CD BioSciences, we offer comprehensive histone modification analysis services in aging, including the analysis of histone methylation, acetylation, phosphorylation, and ubiquitination. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Maleszewska M, et al. Histone Modifications in Ageing and Lifespan Regulation. Curr Mol Bio Rep, 2016, 2: 26-35.