The study of mechanisms associated with cellular senescence and the screenings for pharmacological interventions heavily rely on in vitro models, particularly human primary fibroblasts. While diverse senescence inducers activate some common features, a large variability in the senescence phenotype is observed and dependent on various factors including cell type, stimulus, and time point. Thus, validating cellular senescence models is crucial for ensuring the fidelity and relevance of in vitro systems to the complex in vivo senescent microenvironment.
At CD BioSciences, we understand the critical importance of robust and meticulously validated cellular senescence models in advancing aging-related research and therapeutic development. Our comprehensive suite of cellular senescence model validation services encompasses a spectrum of techniques and assays tailored to elucidate the complex landscape of cellular senescence.
Overview of Our Cellular Senescence Model Validation Services
Senescence marker analysis
Our services encompass an in-depth analysis of several key senescence markers, including:
Analysis methods |
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SA-β-gal staining assay |
Utilizing the established senescence-associated beta-galactosidase (SA-β-gal) staining assay, we meticulously quantify the activity of β-gal, a classic senescence marker, to discern and quantify the population of senescent cells within the model system. |
p16 and p21 expression assessment |
Through advanced molecular techniques such as immunofluorescence and quantitative PCR, we rigorously assess the expression levels of the tumor suppressor proteins p16 and p21, which play instrumental roles in senescence regulation. |
Telomere length measurement |
Leveraging state-of-the-art telomere length measurement assays, we provide precise evaluations of telomere attrition, a hallmark feature of cellular senescence, facilitating a comprehensive characterization of senescent phenotype. |
SASP factor profiling |
We assist clients in profiling the secretion of senescence-associated secretory phenotype (SASP) factors, including pro-inflammatory cytokines, chemokines, and growth factors, to reveal the paracrine influence of the senescent cell and its interplay with the microenvironment. |
Functional assays
In addition to marker analysis, functional assays play a pivotal role in ascertaining the phenotypic and physiological alterations associated with cellular senescence. Our suite of services encompasses a range of functional assays, including but not limited to the following:
Assays |
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Proliferation assays |
We offer comprehensive approaches to assess the proliferative capacity of senescent cells, providing a nuanced understanding of the impact of senescence on cellular replication and growth dynamics. |
Cell cycle analysis |
By employing flow cytometry and advanced imaging techniques, we meticulously analyze the cell cycle profiles of senescent cells, unraveling the intricate alterations in cell cycle progression influenced by the senescent phenotype. |
Migration/invasion assays |
Our services enable the characterization of the migratory and invasive potential of senescent cells, shedding light on the influence of senescence on cellular motility and tissue invasion dynamics. |
Technique Tools Used for Cellular Senescence Model Validation
At CD BioSciences, we are committed to leveraging a wide range of technique tools to ensure the meticulous validation of cellular senescence models.
- High-content imaging systems. By harnessing advanced high-content imaging platforms, we facilitate the comprehensive analysis of senescence markers and functional alterations with high precision and throughput, enabling a detailed phenotypic characterization of senescent cells.
- Next-generation sequencing (NGS). In the cellular senescence model validation, we utilize NGS platforms, such as RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq), to perform in-depth analysis of gene expression profiles, epigenetic modifications, and genomic alterations associated with cellular senescence.
- Proteomic techniques. We employ a series of mass spectrometry-based proteomic techniques, such as liquid chromatography-mass spectrometry (LC-MS) and tandem mass spectrometry (MS/MS), to identify and quantify proteins involved in cellular senescence and the SASP.
At CD BioSciences, we offer comprehensive cellular senescence model validation services, encompassing senescence marker analysis and functional assays. With our dedication to scientific excellence and customer satisfaction, we are your trusted partner in advancing cellular senescence research. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Hernandez-Segura A, et al. Induction and Validation of Cellular Senescence in Primary Human Cells. J Vis Exp, 2018, 136: 57782.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.