The aging population worldwide underscores the urgency for advanced research into the biology of aging and the metabolic processes that accompany it. One such process is gluconeogenesis, the production of glucose from non-carbohydrate sources, which plays a critical role in maintaining blood glucose homeostasis. The pyruvate tolerance test (PTT) is an important tool for assessing the rate of gluconeogenesis using pyruvate as a substrate.
At CD BioSciences, we provide specialized pyruvate tolerance test services in animal models to support aging research. Our services are designed to assess glucose homeostasis, offering invaluable insights for researchers and pharmaceutical companies.
How Do We Perform Pyruvate Tolerance Tests?
- Animal model selection. At CD BioSciences, we begin services by helping clients select appropriate animal models for aging studies. Common models include aging mouse and rat models, which are known for their relevance in mimicking human aging processes. We choose models that allow our clients to investigate the complex interactions between aging and metabolism.
- Fasting. Before testing, animals undergo a defined fasting period to allow blood glucose levels to return to baseline. Our services are essential to ensure accurate interpretation of the pyruvate tolerance test results.
- Pyruvate administration. After fasting, we administer a dose of pyruvate intraperitoneally. This is a critical step, as the dose must be carefully calibrated to ensure results.
- Blood sampling and glucose measurement. We collect blood samples at multiple time points following pyruvate injection, typically at 0 (baseline), 15, 30, 60, and 120 minutes, to measure changes in blood glucose levels.
- Data collection and analysis. Throughout the procedure, our team of experts records data and employs advanced software to evaluate age-related changes in glucose metabolism in animal models.
Our Analysis Services in Pyruvate Tolerance Tests
Glucose metabolism assessment services
Our pyruvate tolerance test services are aimed at helping clients assess glucose metabolism in animal models. By analyzing the changes in blood glucose levels following pyruvate administration, we can infer the efficiency of gluconeogenesis and overall metabolic health. Our services are vital for understanding age-related metabolic disorders and developing potential therapeutic interventions.
Insulin sensitivity analysis services
Age-related insulin resistance is a significant concern in aging research. Our pyruvate tolerance test data helps elucidate the relationship between pyruvate metabolism and insulin action.
Comparative analysis services
Our pyruvate tolerance test services also facilitate comparative studies between different animal models or treatment groups. We provide detailed statistical analyses to support these comparative studies, ensuring that our clients receive actionable and scientifically valid results.
CD BioSciences is committed to advancing the field of aging research through our specialized pyruvate tolerance test services. Our services are designed to support researchers and pharmaceutical companies in their quest to understand and combat age-related metabolic diseases. If you are interested in our services, please feel free to contact us or make an online inquiry.
Reference
- Moro C, Magnan C. Revisited guidelines for metabolic tolerance tests in mice. Lab Anim (NY), 2025, 54 (1): 16-23.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
