Oxidative Stress-Induced Senescence Model Customization Services

Cellular senescence, a cell state characterized by a generally irreversible cell cycle arrest, is implicated in various physiological processes and a wide range of age-related pathologies. Oxidative stress, a condition caused by an imbalance between the production and the elimination of reactive oxygen species (ROS) in cells and tissues, is a common driver of cellular senescence. ROS encompass free radicals and other molecules formed as byproducts of oxygen metabolism, which exhibit varying chemical reactivity.

Fig. 1 Cellular responses to oxidative stress. (Nousis L, et al., 2023)

CD BioSciences is specializing in innovative solutions for aging research. Our expertise lies in the customization of oxidative stress-induced senescence models, allowing our clients to study the intricate mechanisms underlying cellular aging and age-related diseases.

Our Customization Approaches for Inducing Oxidative Stress

Radiation-induced senescence

At CD BioSciences, we employ various techniques to expose cells to ionizing radiation, such as X-rays or gamma rays. Our radiation induction services lead to the generation of free radicals through the ionization of cellular molecules, ultimately resulting in oxidative stress and cellular senescence.

Chemically induced senescence

We offer customization services to induce oxidative stress for cellular senescence by various chemical agents. We utilize hydrogen peroxide (H₂O₂), a common exogenous source of ROS, to mimic oxidative stress conditions. Additionally, our services also employ agents like menadione and tert-butyl hydroperoxide (t-BHP) to disrupt cellular redox homeostasis or mitochondrial function. By customizing the concentration and duration of exposure to these agents, our services facilitate the construction of senescence models tailored to the specific research needs of our clients.

Mitochondrial dysfunction-induced senescence

Mitochondria, as the powerhouse of the cell, are major contributors to ROS production. Our services extend to the customization of cellular models involving the modulation of mitochondrial function to induce senescence. By using mitochondrial inhibitors such as rotenone and antimycin A, we can interfere with the electron transport chain, leading to augmented ROS generation. Our services allow clients to study the impact of mitochondrial dysfunction and oxidative stress on cellular senescence and explore potential interventions.

Workflow of Customizing Oxidative Stress-Induced Senescence Models

Customizing oxidative stress-induced senescence models requires a systematic and well-designed workflow. At CD BioSciences, we follow a comprehensive approach to ensure the generation of reliable models for studying cellular aging.

• Experimental design and cell line selection. Our team of experts assists clients in designing experiments tailored to their needs, considering factors such as cell type and senescence markers of interest. We provide a wide range of cell lines suitable for oxidative stress-induced senescence studies, including primary cells and established cell lines with diverse genetic backgrounds.
• Oxidative stress induction. We employ suitable techniques to ensure controlled and targeted oxidative stress induction. Depending on the chosen approach (radiation, chemical agents, or mitochondrial dysfunction), we optimize the concentration, duration, and timing of the inducers to mimic physiological conditions accurately. Our meticulous approach enhances the relevance and reliability of the resulting senescence models.
• Senescence phenotype analysis. We provide comprehensive analysis services to evaluate the senescence phenotype in the constructed models. We employ a diverse range of techniques, including cell morphology analysis, senescence-associated β-galactosidase (SA-β-gal) staining, cell cycle analysis, and senescence-associated secretory phenotype (SASP) marker measurement.

CD BioSciences excels in providing customized solutions for oxidative stress-induced senescence models. With our expertise, advanced technologies, and strong customer support, we strive to be your trusted partner in advancing scientific knowledge and exploring potential therapeutic interventions for aging-related diseases. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Nousis L, et al. Oxidative Stress-Induced Cellular Senescence: Is Labile Iron the Connecting Link? Antioxidants (Basel), 2023, 12 (6): 1250.