Replicative Senescence-Induced Model Customization Services

Replicative senescence involves an irreversible arrest of cell proliferation and altered cell function. It is controlled by several dominant-acting genes and depends on the number of cell divisions, not on time. It also depends on the cell type, species, and age of the donor. The replicative senescence-induced models are valuable for studying cellular senescence. Researchers can explore the molecular mechanisms that drive senescence to identify potential targets for intervention. This model allows researchers to screen and develop compounds that modulate senescence pathways.

Fig. 1 The route to replicative senescence. (Pizzul P, et al., 2023)

At CD BioSciences, we specialize in providing comprehensive customization services for replicative senescence-induced models, tailored to meet the unique needs of our clients. Leveraging our extensive experience and cutting-edge technologies, we are committed to supporting the scientific community in advancing our understanding of aging and developing potential interventions.

Customization Options for Replicative Senescence-Induced Models

Cell line selection

We assist our clients in selecting suitable cell lines for their specific research objectives. Our extensive knowledge and database of cell lines enable us to recommend cell lines that closely mimic the senescence patterns.

Culture conditions

We offer customized cell culture protocols to create an environment that promotes or delays cellular senescence, depending on the experimental goals. Our team of experts carefully designs culture media compositions, growth factors, and culture conditions to ensure the desired senescence phenotype is achieved. By modulating factors such as oxygen levels, nutrient availability, and pH, we can create tailored culture conditions that closely mimic the in vivo aging environment.

Passage intervals

We guide passage intervals to ensure the desired number of cell divisions and senescence progression. We can synchronize the senescence process by optimizing the timing of cell subculturing, enabling clients to study specific stages of replicative senescence in a controlled and reproducible manner.

Senescence marker selection

We offer comprehensive assays to measure various senescence-associated markers, including senescence-associated β-galactosidase (SA-β-gal) activity, p16^INK4a expression, telomere length, and DNA damage markers. Our analysis services help clients delve into the cellular and molecular changes associated with replicative senescence.

Workflow of Customizing Replicative Senescence-Induced Models

At CD BioSciences, we follow a systematic workflow to help clients customize replicative senescence-induced models, ensuring high-quality results.

• Cell line expansion. After optimizing culture conditions and passages, we expand the selected cell lines. We culture cells at the defined passage intervals to allow for successive rounds of cell division. This process is repeated until the cells reach replicative senescence or the desired experimental endpoint.
• Cell growth monitoring. Throughout the model construction, we closely monitor cell growth and proliferation. Our services include regular cell counting, viability assessment, and morphological observations. By tracking the growth kinetics, we assist clients in evaluating the replicative lifespan and senescence progression of the cells.
• Assessment of senescence markers. We assess specific senescence markers to validate replicative senescence-induced models,
• Data analysis and interpretation. Once the aging cell models are established and the experiments are completed, we perform data analysis and interpretation. Our team of experts provides comprehensive reports and assists in interpreting the results.

CD BioSciences offers a wide range of customization services for replicative senescence-induced models to facilitate aging research. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Pizzul P, et al. The multistep path to replicative senescence onset: zooming on triggering and inhibitory events at telomeric DNA. Front Cell Dev Biol, 2023, 11: 1250264.