Oncogene-Induced Cellular Senescence Model Customization Services

Oncogene-induced senescence (OIS) is a robust and sustained antiproliferative response brought about by oncogenic signaling resulting from an activating mutation of an oncogene, or the inactivation of a tumor-suppressor gene. Oncogene-induced cellular senescence modeling can aid in the identification of potential therapeutic targets to modulate senescence responses in cancer cells.

Fig. 1 OIS in complex biological systems. (Galluzzi L, Vitale I, 2018)

CD BioSciences is dedicated to providing cutting-edge services in the field of aging research. Our expertise lies in offering customized solutions for constructing oncogene-induced cellular senescence models.

Oncogene-Induced Cellular Senescence Model Customization Options

Oncogene selection

Our team of experts assists clients in navigating through the vast array of oncogenes available and selecting those that align with the client's research objectives. For instance, oncogenes like H-RAS, K-RAS, and BRAF are commonly used to induce senescence in various cell types.

Cell line selection

Different cell lines exhibit varying susceptibilities to OIS and selecting a cell line that closely mimics the biological context of your study is essential. We assist our clients in evaluating the available options and choosing a cell line that best aligns with the research goals. Whether human fibroblasts, epithelial cells, or immortalized cell lines, we have the expertise to guide clients in making an informed decision.

Gene delivery methods

With our diverse gene delivery methods, we can customize the approach to suit the client's experimental needs and maximize the success of your oncogene-induced cellular senescence models.

Workflow of Customizing Oncogene-Induced Cellular Senescence Models

• Experimental design and execution. Our team of experts will collaborate with clients to develop experimental designs that are tailored to the specific research objectives. We assist in determining the optimal timing of oncogene induction, dosing, and monitoring parameters. Furthermore, we help clients establish appropriate control groups to ensure the validity of the client's experimental results. Leveraging our extensive experience in experimental design and execution, we can help clients construct oncogene-induced cellular senescence models.
• Phenotypic characterization. We provide comprehensive support in phenotypic characterization, enabling clients to delve into the cellular changes associated with senescence. We offer a wide range of assays and techniques to evaluate the effects of OIS, such as senescence-associated β-galactosidase (SA-β-gal) staining, senescence-associated secretory phenotype (SASP) analysis, telomere length analysis, cell proliferation assays, cell cycle analysis.
• Data analysis and interpretation. We assist clients in analyzing your data, identifying statistically significant differences between experimental groups and controls, and visualizing the results clearly and concisely.

CD BioSciences offers oncogene-induced cellular senescence model customization services, providing tailored solutions for clients in the field of aging biology. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Galluzzi L, Vitale I. Oncogene-induced senescence and tumor control in complex biological systems. Cell Death Differ, 2018, 25 (6): 1005-1006.