Analysis Services for Aging Proteostasis Decline

INQUIRY

Protein homeostasis also referred to as proteostasis, is essential for preserving cellular function and preventing the onset of numerous ailments. Nonetheless, proteostasis decreases during the aging process, resulting in the buildup of misfolded and accumulated proteins. This phenomenon is linked to the onset of age-related illnesses including neurodegenerative disorders, cardiovascular diseases, and cancer.

Fig. 1 Overview of the proteostasis network (PN). (Labbadia J and Morimoto RI, 2015)

CD BioSciences offers comprehensive analysis services specifically designed to investigate the loss of protein homeostasis in aging. We are proficient in systems biology approaches and network modeling to understand the interconnected pathways and regulatory mechanisms governing protein homeostasis during aging.

Overview of Our Loss of Proteostasis Analysis Services

Our Analysis Services	Details
Protein aggregation assays	We conduct tests to evaluate the aggregation tendency of proteins associated with aging. These may incorporate approaches like thioflavin T assays, sedimentation assays, or dynamic light scattering to quantify protein aggregation.
Chaperone activity assays	We assess the activity of molecular chaperones involved in protein folding and proteostasis maintenance, using methods to measure ATPase activity, substrate binding, and refolding activity of chaperone proteins.
Proteostasis network analysis	We provide a comprehensive analysis of the proteostasis network utilizing techniques such as mass spectrometry-based proteomics to identify changes in protein turnover, degradation pathways, and post-translational modifications associated with aging.
Protein quality control pathway assessments	We evaluate the ubiquitin-proteasome system and autophagy pathways through assays that assess proteasome activity, autophagic flux, and lysosomal function in the context of aging-related protein homeostasis.
Heat shock response profiling	We perform profiling of the heat shock response and heat shock protein expression in aged tissues or cells, possibly using quantitative Western blotting, immunofluorescence imaging, or gene expression analysis.

Workflow of Analyzing Loss of Proteostasis During Aging

Sample acquisition
We obtain tissue samples or cultured cells from aged individuals or animal models representing different stages of aging. In some cases, we select appropriate model organisms (e.g., yeast, worms, flies, mice) for the identification of specific cellular pathways or proteostasis mechanisms.
Protein extraction, quantification, and experimental assays
We extract proteins from the samples using appropriate buffers and techniques to obtain a representative proteome. Then, we quantify the total protein content using methods such as the BCA assay or Bradford assay. Then, CD BioSciences carries out experiments to collect data on the changes in protein homeostasis (proteostasis) during aging.
Data integration and analysis
We integrate the results from different analytical techniques and perform bioinformatic analyses to identify age-related alterations in proteostasis pathways, potentially leading to the identification of novel targets or pathways for intervention.

Our Advantages

We have a team of experts spanning molecular biology, biochemistry, aging research, and bioinformatics to approach the complexities of protein homeostasis from various angles, ensuring a holistic understanding of the aging process.
Our experience and facilities for working with model organisms such as yeast, worms, flies, and mice are essential for conducting in vivo studies to understand the impact of aging on protein homeostasis at the organismal level.

CD BioSciences aims to unravel the mechanisms underlying the loss of protein homeostasis in aging and contribute to the development of therapeutic strategies to mitigate age-related diseases. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

Labbadia J, Morimoto RI. The biology of proteostasis in aging and disease. Annu Rev Biochem. 2015, 84: 435-64.

All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.