Reactive oxygen species (ROS) comprise various highly reactive molecules such as superoxide anion (O2-), hydroxyl radical (•OH), and hydrogen peroxide (H2O2). Their propensity to cause oxidative degradation of DNA, protein, and lipid makes ROS a likely contributor to the aging process. CD BioSciences offers advanced analysis services that aim to clarify the molecular mechanisms underlying mitochondrial dysfunction mediated by ROS. Our services provide valuable insights into potential therapeutic interventions.
Overview of ROS in Mitochondrial Dysfunction Analysis Services
Our ROS in mitochondrial dysfunction analysis services encompass a wide range of techniques and methodologies to assess ROS production, oxidative damage, mitochondrial function, and cellular responses. These services include, but are not limited to the following.
Measurement of ROS levels
We employ various assays to quantify ROS levels in different cellular models or tissues. Fluorescent probes, such as dihydroethidium (DHE) or 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA), are utilized to detect and quantify intracellular ROS generation. By comparing ROS levels between young and aged samples, we can evaluate the impact of ROS on mitochondrial dysfunction during aging.
Assessment of oxidative damage
Through techniques like immunohistochemistry, immunoblotting, and mass spectrometry, we evaluate the extent of oxidative modifications, such as DNA strand breaks, protein carbonylation, and lipid peroxidation. Our analysis services provide insights into the impact of ROS on cellular macromolecules and mitochondrial function.
Evaluation of mitochondrial function
We utilize various approaches to assess mitochondrial parameters, including mitochondrial membrane potential, ATP production, electron transport chain activity, and mitochondrial dynamics. By examining these functional aspects, we gain a comprehensive understanding of how ROS contributes to mitochondrial dysfunction during aging.
Analysis of the Relationships Between Mitochondria, ROS, and Aging
Analysis of the effects of redox stress signaling threshold (RST) on aging
We utilize animal models, such as C. elegans, to study the effects of altering RST on aging-related phenotypes, involving genetic manipulation, pharmacological interventions, or environmental stressors to modulate redox status and observe the resultant impact on aging.
Analysis of the effects of mitochondrial depolarization on aging
We examine the impact of mitochondrial depolarization on mitochondrial ROS production in various tissues, including mice, naked mole rats (NMRs), and bats, and assess its effect on aging. Based on these studies, we have also helped our clients develop mitochondrial mild depolarization for anti-aging solutions.
Workflow of Analyzing ROS in Mitochondrial Dysfunction During Aging
- Sample collection
We work closely with our clients to determine the appropriate sample types, including tissues, cells, or animal models, for their specific aging research needs.
- Sample processing and analysis
We process the collected samples to extract relevant biomolecules for downstream analysis. The extracted material undergoes a series of assays, including ROS measurement, oxidative damage assessment, mitochondrial function evaluation, and analysis of cellular responses to ROS.
- Data analysis and interpretation
We offer in-depth data analysis and interpretation, utilizing advanced bioinformatics tools and statistical methods. Our analysis services go beyond generating raw data. We provide comprehensive reports that include detailed statistical analyses, visualizations, and clear interpretations of the results.
CD BioSciences provides precise analysis services for investigating the function of ROS in mitochondrial dysfunction throughout the aging process. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
