Aging is characterized by a decline in organismal functions and a range of key hallmarks, comprising genetic and epigenetic alterations. These epigenetic changes linked with aging incorporate DNA methylation, histone modification, chromatin remodeling, regulation of non-coding RNA (ncRNA), and RNA modification. All of these processes contribute to the regulation of aging and consequently promote age-related diseases. CD BioSciences specializes in providing comprehensive analysis services for epigenetic alterations during aging.
Overview of Our Epigenetic Alterations Analysis Services
CD BioSciences offers a wide range of analysis services dedicated to studying epigenetic alterations during aging. Our services encompass various aspects of epigenetic modifications, including DNA methylation, histone modification, chromatin remodeling, ncRNA regulation, and RNA modification, providing our clients with a comprehensive understanding of the regulatory mechanisms involved in aging and age-related diseases.
Applications of Analyzing Epigenetic Alterations in Aging Studies
Identification of aging biomarkers
We identify epigenetic alterations as valuable biomarkers of aging, providing insights into the biological age of an individual, which may differ from chronological age. DNA methylation patterns, histone modifications, and non-coding RNAs can be utilized as biomarkers to assess the aging process and age-related diseases. The following table shows the markers we support for scientific research.
| Epigenetic changes with aging |
Markers |
Models |
| Reduced global heterochromatin |
HP1 and H3K9me3 reduced, lamin A changes |
C. elegans, Drosophila, human fibroblasts, progeria patient cells. |
| Nucleosome remodeling and loss |
Loss of core histone proteins |
Yeast, worms, human fibroblasts. |
| Histone variants increased |
H3.3, H3.3cs1 |
Mouse brain, human fibroblasts. |
| Changed histone markers |
Globally active markers increased (H3K4me3, H4K16ac) and repressive marks decreased (H3K9me3, H3K27me3) |
Yeast, C. elegans, Drosophila, killifish, rats, mouse brain, mouse stem cells, mouse fibroblasts, etc. |
| DNA methylation changes |
Global DNA hypomethylation, CpG island hypermethylation |
Salmon, mice, rats, dogs, rhesus monkeys, human stem cells. |
Analysis of tissue-specific aging
Epigenetic changes may vary among tissues and cell types in an organism. Our analysis of these specific tissue alterations offers insight into the heterogeneity of aging within an individual and the varying susceptibility of organs to dysfunction related to aging.
Longitudinal studies
By examining changes in epigenetic marks over time, we gain a deeper understanding of the trajectory of aging and age-related diseases, potentially leading to the identification of critical intervention points.
Workflow of Analyzing Epigenetic Alterations During Aging
- Sample collection
We offer guidance on sample collection and preparation to ensure the integrity and quality of the biological material. Whether it concerns DNA, histones, chromatin, or RNA, our experts provide optimized protocols and handle samples with utmost care to minimize variability and ensure reproducibility.
- Experimental assays
We employ a range of experimental techniques to analyze epigenetic modifications. These include next-generation sequencing (NGS) technologies, chromatin immunoprecipitation (ChIP), bisulfite sequencing, RNA sequencing (RNA-seq), and various other molecular biology assays. Our experienced scientists tailor the experimental assays based on the specific research needs, allowing for comprehensive analysis of epigenetic alterations during aging.
- Results and reporting
We provide comprehensive and detailed reports summarizing the results of the epigenetic analysis during aging. The reports include visual representations of the data, such as heatmaps, genome browser tracks, and gene ontology enrichment analysis.
CD BioSciences employs various molecular biology assay platforms and robust bioinformatics tools to guarantee precise and comprehensive examination of epigenetic changes during the aging process. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
