Studying epigenetic regulation at the single-cell level has advanced comprehension of distinct cellular states and mechanisms preserving them. Investigating epigenomic profiles of aging cells delivers crucial comprehension of the processes responsible for aging and age-related diseases. Currently, various techniques for single-cell epigenomic sequencing have been devised. CD BioSciences offers epigenomic profiling services, encompassing the examination of DNA methylation, histone modifications, DNA accessibility, and chromosome conformation.
Overview of Our Single-Cell Epigenomic Profiling Services
Analysis of DNA modifications in aging
Our single-cell epigenomic profiling services enable the analysis of DNA modifications, such as DNA methylation, at the single-cell level. By examining the DNA methylation patterns of individual cells, we can identify age-associated changes and potential biomarkers of aging.
Analysis of histone modifications in aging
We can identify age-related changes in chromatin states and uncover the epigenetic mechanisms driving aging processes, by examining the histone modification profiles of individual cells. This information helps elucidate the role of histone modifications in aging and provides potential targets for therapeutic interventions.
DNA accessibility analysis in aging
Changes in DNA accessibility, which determine the accessibility of genomic regions to transcription factors and other regulatory proteins, have been implicated in aging and age-related diseases. By examining the open chromatin regions in individual cells, we can identify age-related changes in gene regulatory elements and gain insights into the functional consequences of altered DNA accessibility during aging.
Chromosome conformation analysis in aging
Our single-cell epigenomic profiling services include the analysis of chromosome conformation in aging single cells. By examining the spatial organization of chromosomes at the single-cell level, we can uncover age-related changes in genome architecture and understand how alterations in chromosome conformation contribute to aging and age-related diseases.
Technology Platforms for Single-Cell Epigenomic Profiling
At CD BioSciences, we employ advanced technology platforms to perform single-cell epigenomic profiling, ensuring the generation of high-quality and comprehensive data.
Single-cell bisulfite sequencing (scBS-seq)
In scBS-seq, we treat genomic DNA with bisulfite, converting unmethylated cytosines to uracils while leaving methylated cytosines unchanged. So, we can determine the DNA methylation status of individual cells. This technology provides a high-resolution view of DNA methylation patterns, allowing for the identification of age-related changes and the characterization of cell-to-cell variability in DNA methylation.
Single-cell chromatin immunoprecipitation sequencing (scChIP-seq)
We utilize scCHIP-seq to analyze histone modifications and protein-DNA interactions in individual cells. By this method, we can identify age-related changes in histone modifications and gain insights into the epigenetic regulation of aging.
Assay for transposase-accessible chromatin using sequencing (scATAC-seq)
We use a transposase enzyme to insert sequencing adapters into accessible regions of the genome, to identify age-related changes in DNA accessibility and uncover regulatory elements associated with aging.
Single-cell chromosome conformation capture sequencing(scHi-C)
We can investigate the three-dimensional organization of the genome at the single-cell level, by capturing and sequencing the interactions between chromatin segments. This technology provides insights into the structural changes in chromatin architecture that occur during aging.
Our Advantages
- We adhere to strict quality control measures throughout the entire workflow, to generate high-quality data to support aging research.
- We offer customized solutions tailored to meet specific research needs. Our team works closely with clients to design experimental strategies and optimize protocols throughout the project.
Based on robust research platforms, CD BioSciences enables our clients to unravel the epigenomic landscape of aging single cells and advance the field of aging biology. If you are interested in our services, please feel free to contact us or make an online inquiry.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
