Whole Genome Bisulfite Sequencing (WGBS)

Changes in methylation levels may be linked to the occurrence of aging and the development of age-related diseases, so the detection and quantification of methylation is crucial for understanding age-related gene expression and epigenetically regulated processes. As a high-quality aging and longevity research service provider, CD BioSciences provides Whole Genome Bisulfite Sequencing (WGBS) to efficiently identify and analyze DNA methylation on a genome-wide scale.

Why Choose WGBS

Whole-genome bisulfite sequencing (WGBS) is a bisulfite sequencing method that can be used to detect deep DNA methylation across the genome. This method refers to the detection of methylated cytosines by treating DNA samples with sodium bisulfite prior to sequencing. The specific principle is that after the genomic DNA is treated with sodium bisulfite, the unmethylated cytosine in the DNA is converted into uracil, while the methylated cytosine remains intact. After sample processing, steps such as PCR amplification, library preparation, and sequencing are performed. Finally, comparing the sequencing data of untreated and treated DNA sequences can identify those sites that are methylated. WGBS provides a scalable solution for high-throughput DNA methylation analysis in aging and longevity studies.

Our Services

We use a professional technology platform for high-throughput sequencing, and we provide genome-wide DNA methylation analysis services that can fully meet each customer's specific requirements and cost budgets in DNA methylation exploration. Specific services include:

service items	Specific requirements and operations
Sample preparation	Sample sources can include humans, animals, etc., and can provide samples in the form of DNA, cells, and tissues.(DNA＞20 ng/µl, quantity≥250 ng; cells＞50 ng/µl, quantity≥0.2-1 M; tissue＞50 ng/µl, quantity≥5 mg） Sample preparation includes operations such as genomic DNA extraction, purification, quantification, and quality control.
Sequencing	Base-level detailed sequencing of entire genomes. We provide all genome-wide bisulfite sequencing data and plots, including methylation heatmaps, CpG methylation distributions, DNA methylation average reads, and more.
Data analysis	We provide free technical support to assist with data analysis. Specific analysis may include differential methylation annotation, single CpG site methylation analysis, alignment analysis with reference genomes, etc.

Technical Route

The specific process of our service mainly includes three steps, and the specific technical route is as follows.

In order to ensure the accuracy and reliability of the data, we carry out strict quality control on each experimental step, and finally can carry out a correct and comprehensive bioinformatics analysis.

Advantages

• Cover every CpG site and non-CpG site methylation in the complete genome.
• Comprehensive bioinformatics analysis enables read mapping, methylation calling and differential methylation analysis of raw data.
• A full and fast service cycle enables the fastest turnaround time to report results based on the number of samples.

CD BioSciences can solve DNA methylation analysis problems in aging-related research, such as whole gene coverage, differential methylation, and identification of new CpG sites. We provide the shortest path to genome-wide methylation analysis. If you have any questions, please feel free to contact us.

Our services are for research use only and not for any clinical use.