Adipose tissue (AT) is not only a passive energy reservoir but also an active endocrine organ involved in systemic metabolic regulation, immunomodulation, and thermogenesis. With aging, adipose tissue undergoes extensive remodeling characterized by a progressive decline in regenerative capacity, increased inflammation, and dysfunction in lipid and glucose metabolism.
Fig. 1 The age-related redistribution of adipose tissue. (Noronha IL, et al., 2022)
At CD BioSciences, we offer a full spectrum of services to characterize adipose tissue senescence, enabling clients to uncover the mechanisms of aging-related adipose tissue dysfunction and to accelerate the development of targeted therapeutic strategies.
Characterization of Cell Senescence in Adipose Tissue
At CD BioSciences, we specialize in the comprehensive characterization of cellular senescence across key cell types in adipose tissues, including adipose progenitor cells (APCs) and microvascular endothelial cells. These cells play pivotal roles in maintaining adipose tissue homeostasis, and their senescence contributes to aging-related adipose tissue dysfunction.
Using senescence-associated β-galactosidase (SA-β-gal) staining and flow cytometry, we can help clients quantify senescent cells in the stromal vascular fraction (SVF) from adipose tissue. We also analyze the expression of key senescence markers using quantitative RT-PCR and immunocytochemistry. In addition, we assess transcriptional regulators of adipogenesis to evaluate how senescence impairs lineage commitment. We also employ immunostaining for endothelial markers combined with senescence markers to characterize cell senescence within the microcirculation of adipose tissue.
Characterization of Age-Related Changes in Adipose Tissue in Animal Models
CD BioSciences supports adipose tissue senescence research through the use of well-characterized rodent and non-human primate models. We offer advanced imaging, histological, molecular, and functional assays to assess adipose tissue senescence.
Characterization of age-related redistribution of adipose tissue
A hallmark of aging is the redistribution of fat from subcutaneous to visceral compartments, a shift closely linked to metabolic disease and chronic inflammation. We use magnetic resonance imaging (MRI), computed tomography (CT), and high-resolution ultrasound imaging to quantify age-related redistribution of adipose tissue, including measuring fat mass and performing histological analysis of adipocyte cell size and morphology. We also offer immunophenotyping services to characterize inflammation and immune cell infiltration in different adipose depots, helping clients analyze the association of cellular senescence with depot-specific adipose inflammation and metabolic diseases.
Characterization of age-related impairment of brown adipocyte development and function
During aging, brown adipose tissue (BAT) mass and activity decline due to impaired differentiation of brown preadipocytes and mitochondrial dysfunction in mature brown adipocytes. We offer histological analysis to assess BAT morphology and lipid accumulation, enabling evaluation of adipose tissue architecture and fat content. Our company also provides immunostaining targeting key differentiation markers to determine the differentiation status of brown adipocytes. We also offer services to measure oxygen consumption rate (OCR), mitochondrial membrane potential, and reactive oxygen species (ROS) production, providing insights into age-associated mitochondrial impairment in BAT.
Adipose tissue senescence is a central driver of age-related metabolic dysfunction and inflammation. CD BioSciences offers an integrated suite of services to characterize adipose tissue senescence at the molecular, cellular, and organismal levels. If you are interested in our services, please feel free to contact us or make an online inquiry.
References
- Ou MY, et al. Adipose tissue aging: mechanisms and therapeutic implications. Cell Death Dis, 2022, 13 (4): 300.
- Nerstedt A, Smith U. The impact of cellular senescence in human adipose tissue. J Cell Commun Signal, 2023, 17 (3): 563-573.
All of our services and products are intended for preclinical research use only and cannot be used to diagnose, treat or manage patients.
