IRS-1 (D5T8J) Rabbit Antibody
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IRS-1 (D5T8J) Rabbit Antibody

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CAT. NO.: AMA-0012

Target Information
UniProt ID P35568
Product Details
Size 100 μL
Reactivity Human, Mouse, Rat
Host Rabbit
Source/Isotype Rabbit IgG
Storage Store at -20°C.
Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His653 of human IRS-1 protein.
Usage
Application WB, IP
Product Usage Information Dilution Western Blotting (1:1000); Immunoprecipitation (1:200)
MW (Target) 180 kDa
Background
Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.

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