2′,7′-Dichlorofluorescein

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CAT. NO.: AF018

Product Details
Synonym(s)	Dichlorofluorescein, DCF
Empirical Formula (Hill Notation)	C₂₀H₁₀Cl₂O₅
CAS Number	76-54-0
Molecular Weight	401.2
MDL Number	MFCD00005047
PubChem Substance ID	57649439
Size	5 g/25 g
Assay	≥90% (T)
Fluorescence	λ_ex 504 nm; λ_em 529 nm in 0.1 M Tris pH 8.0
SMILES string	Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

Description
2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Description

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application
2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. (3) It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

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