Customization Services for Senescent Fibroblasts

The development of therapeutic approaches to slow or reverse the adverse physiological and pathological changes associated with aging has been considered an important goal of gerontological research. As cellular senescence has been characterized as the basis of aging in organisms, the culture and subculture of normal human diploid fibroblasts to mimic in vivo aging processes has been developed as an important method to study the molecular events involved in aging.

Fig. 1 Schematic illustration of senescent fibroblasts. (Noren Hooten N and Evans MK, 2017)

CD BioSciences, a pioneer in aging research, offers a range of customization services for senescent fibroblasts, providing our clients with valuable tools to unravel the mechanisms underlying aging and explore potential anti-aging strategies.

Modeling Methods for Senescent Fibroblasts

At CD BioSciences, we employ various methods to induce senescence in fibroblasts, faithfully recapitulating the aging process in vitro. By simulating the key hallmarks of aging, our customized services enable clients to study the complex interplay of cellular and molecular events associated with aging.

UVB irradiation of fibroblast

UVB radiation triggers DNA damage and oxidative stress, activating cellular senescence pathways. We utilize controlled UVB irradiation to induce senescence in fibroblasts, contributing to the study of the process of photoaging in the skin. Our approach provides researchers with a valuable tool to investigate the impact of UV radiation on fibroblast aging and study the molecular mechanisms involved.

Accelerated proliferation of fibroblast

We provide services for constructing senescent fibroblast models by inducing cell proliferation. By culturing fibroblasts with optimized media for rapid cell division, we can induce replicative senescence more efficiently. Our approach allows clients to study the effects of accelerated aging on fibroblast behavior and explore potential interventions to counteract premature aging.

In vitro H₂O₂ induction

Our services help clients construct the H₂O₂-induced premature senescence model effectively with appropriate cell-line type, maintaining procedures, dose, time of exposure, and type of solvent. We treat fibroblast cultures with varying concentrations of H₂O₂ for specific durations to mimic oxidative stress conditions found in aging cells.

How Do We Customize Aging Fibroblasts?

We follow a streamlined workflow to provide high-quality and customized services for culturing aging fibroblasts. Our workflow ensures reproducibility, accuracy, and efficiency in generating aged fibroblast models for research purposes.

• Fibroblast isolation. We collect and process skin tissue samples or other relevant sources meticulously to obtain pure fibroblast populations.
• Culturing and senescence induction. We apply our specialized methods such as UVB irradiation or accelerated proliferation techniques to induce senescence. This step ensures the development of an aging fibroblast population for downstream analyses.
• Senescence analysis. We perform comprehensive senescence analysis on the cultured fibroblasts, including assessing senescence markers such as β-galactosidase activity, telomere length, DNA damage accumulation, and gene expression patterns associated with senescence.
• Functional assays. CD BioSciences offers a range of functional assays to evaluate various aspects of aged fibroblast behavior. Our assays include assessing cell proliferation rates, migration capacity, secretory profile (senescence-associated secretory phenotype, SASP), and response to stimuli.
• Genetic analysis. Our services also encompass genetic analysis of aging fibroblasts. Using techniques such as whole-genome sequencing, targeted gene panels, or epigenetic profiling, we can identify genetic variations or mutations associated with aging in fibroblasts. Our analyses contribute to a deeper understanding of the genetic factors influencing the aging process.

Utilizing a range of techniques to induce senescence and monitor the aging process, CD BioSciences provides clients with aging fibroblasts as valuable tools to advance the understanding of aging biology. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

1. Noren Hooten N, Evans MK. Techniques to Induce and Quantify Cellular Senescence. J Vis Exp. 2017, (123): 55533.
2. Lago JC, Puzzi MB. The effect of aging in primary human dermal fibroblasts. PLoS One, 2019, 14 (7): e0219165.
3. Chen H, et al. Cell senescence culturing methods. Methods Mol Biol. 2013, 1048: 1-10.