Customization Services for Aging Myoblasts

Myogenesis gradually deteriorates as the skeletal muscle ages, contributing to muscle mass loss. Indeed, one of the most serious consequences of aging is the progressive loss of muscle mass (sarcopenia) and function, which affects the quality of life and, ultimately, the survival of elderly people.

CD BioSciences specializes in providing a range of services for aging myoblasts. Our expertise lies in inducing senescence in myoblasts, detecting aging markers, and performing comprehensive analyses to assess the effects of myoblast aging on different factors.

Modeling Methods for Aging Myoblasts

Culture with serum from the elderly

Studies have shown that factors in the serum of aged individuals can influence the aging process in cells. We subject myoblasts to a microenvironment resembling that of an aged individual to mimic the physiological conditions and induce senescence. Our method allows us to help clients investigate the effects of aging factors present in the serum on myoblast behavior, gene expression, and cellular functions.

Microgravity induction of myoblasts

We also employ microgravity induction techniques to study the effects of gravity on myoblast aging. Our specialized equipment allows clients to the creation of controlled microgravity conditions, enabling the study of changes in myoblast morphology, gene expression, protein synthesis, and cellular signaling pathways.

How Do We Detect Aging in Myoblasts?

Accurate detection of aging in myoblasts is essential for evaluating the efficacy of interventions and monitoring the progression of age-related changes. CD BioSciences employs various techniques to detect the expression of major hallmarks of cellular including the expression of senescence-associated beta-galactosidase (SA-β-gal), size, morphology, proliferation, DNA damage (γH2AX), and histone modifications (H3K9me3 and H3K27me3), enabling precise characterization and quantification of age-related changes.

Our Services for Analyzing the Effects of Aging Myoblasts on Factor Expression

CD BioSciences offers comprehensive analysis services to assess the effects of myoblast aging on various factor expression levels. We analyze the expression of key regulatory factors to assist clients in uncovering the molecular changes associated with myoblast aging and identify potential targets for therapeutic interventions.

• Analysis of effects of myoblast aging on the expression of myogenic regulatory factors (MRFs). We offer analysis services to evaluate the expression of MRFs in aging myoblasts. By comparing the expression levels of MRFs in young and aged myoblasts, we help clients identify alterations that may contribute to age-related muscle decline.
• Analysis of effects of myoblast aging on the expression of muscle atrophy factors. We conduct analysis services to assess the expression of muscle atrophy factors in aging myoblasts. We examine the levels of key factors involved in muscle protein degradation pathways, to help clients uncover the molecular changes associated with myoblast aging and identify potential targets for interventions aimed at preventing or reversing muscle atrophy.

Combining various advanced techniques, rigorous analysis, and a deep understanding of myoblasts, CD BioSciences is committed to advancing the field of aging myoblast customization. If you are interested in our services, please feel free to contact us or make an online inquiry.

Reference

1. Moustogiannis A, et al. The Effects of Muscle Cell Aging on Myogenesis. Int J Mol Sci, 2021, 22 (7): 3721.