Customization Services for Aging Endothelial Cells

The endothelial cell (EC) monolayer forms the inner cellular lining of all blood vessels forming a critical interface between blood and tissue. Vascular endothelium is involved in physiological functions, which include regulation of blood fluidity, hemostasis and clotting, vascular tone, immune responses, inflammation, angiogenesis, and metabolism. Modeling aging ECs mimics the aging process in a controlled laboratory setting, enabling researchers to study age-related changes without the confounding factors present in in vivo models.

Fig. 1 Characteristics of senescent endothelial cells. (Hwang HJ, et al., 2022)

CD BioSciences is a leading company specializing in providing customization services for aging ECs. We offer tailored solutions to meet the unique research needs of scientists and researchers studying age-related diseases and the cellular mechanisms underlying EC senescence.

Modeling Methods for Aging ECs

Induction of oxidative stress in ECs

At CD BioSciences, we have developed robust approaches to induce oxidative stress in ECs for studying the aging process. We carefully calibrate the concentration and exposure time of H₂O₂ to induce controlled levels of oxidative stress in ECs, which allows for the investigation of the molecular and cellular changes associated with senescence.

Induction of replicative senescence in ECs

With each cell division, telomeres gradually shorten until a critical threshold is reached, triggering cell cycle arrest and cellular senescence. We offer services for the long-term culture of ECs for induction of replicative senescence. Our approach enables clients to study telomere shortening in aging ECs and their implications in age-related diseases.

Induction senescence via Inflammatory signals in ECs

Inflammatory cytokine can stimulate the senescence-associated secretory phenotype (SASP) in ECs. This secretory phenotype involves the secretion of various factors that perpetuate senescence and contribute to tissue dysfunction. We offer customized services for exposing ECs to pro-inflammatory signals, such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), allowing clients to investigate the molecular mechanisms involved in inflammatory-induced senescence and its implications in age-related diseases.

Induction of mechanical stress in ECs

We provide services for subjecting ECs to controlled mechanical stress, such as high fluid shear stress or cyclic stretch. Our approaches can activate signaling pathways related to oxidative stress, inflammation, and mechanotransduction, ultimately leading to endothelial cell senescence.

Applications of Aging Endothelial Cell Models in Aging-Related Diseases

Endothelial cell senescence is implicated in several age-associated conditions, including atherosclerosis, vascular aging, and cardiovascular diseases. We provide customized aging endothelial cell models to help clients investigate the molecular and cellular changes that contribute to disease development and progression. Our aging models also serve as platforms for testing potential therapeutic interventions targeting endothelial cell senescence.

• In atherosclerosis research, we use aging endothelial cell models to help clients unravel the mechanisms underlying endothelial dysfunction, plaque formation, and inflammation.
• In cardiovascular diseases, we employ aging endothelial cell models to offer the opportunity to explore therapeutic strategies that target senescent endothelial cells and promote tissue regeneration.

Modeling aging endothelial cells is a powerful approach to studying the cellular and molecular mechanisms underlying age-related diseases. CD BioSciences' customization services for inducing senescence in ECs through various methods provide researchers with valuable tools to advance our understanding of these diseases. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

1. Hwang HJ, et al. Factors and Pathways Modulating Endothelial Cell Senescence in Vascular Aging. Int J Mol Sci, 2022, 23 (17): 10135.
2. Ting KK, et al. The aging endothelium. Vasc Biol, 2021, 3 (1): R35-R47.