Customization Services for Aging Epithelial Cells

Epithelial cells play a critical role in maintaining the integrity and function of various tissues and organs in the body. As these cells age, their functionality and regenerative capacity decline, leading to impaired tissue homeostasis and increased susceptibility to disease. Modeling senescence in epithelial cells is of paramount importance for understanding the underlying mechanisms and developing targeted interventions to promote healthy aging and prevent age-related diseases.

CD BioSciences is a leading company specializing in providing high-quality customization services for aging epithelial cells. With years of experience in the industry, we have developed a deep understanding of the unique challenges and requirements associated with studying aging in epithelial cells.

Modeling Methods for Aging Epithelial Cells

Oxidative stress induction of aging epithelial cells

Oxidative stress leads to cellular damage and triggers a cascade of events that ultimately culminate in senescence. To model aging epithelial cells, we employ various techniques and compounds that induce oxidative stress.

Doxorubicin induction of aging epithelial cells

In addition to oxidative stress induction, we also utilize doxorubicin, a chemotherapeutic agent, to induce senescence in epithelial cells. Our experienced team of scientists at CD BioSciences has optimized the doxorubicin induction protocols to ensure reproducibility and minimize cell-to-cell variability. Our services allow for an accurate and reliable assessment of the aging phenotype in cultured epithelial cells.

How Do We Monitor the Aging Process in Epithelial Cells?

At CD BioSciences, we employ a combination of molecular and cellular markers to assess the aging phenotype and track the progression of senescence in cultured epithelial cells.

• One commonly used marker is senescence-associated β-galactosidase (SA-β-gal), an enzyme that is upregulated in senescent cells. By performing SA-β-gal staining, we can visualize and quantify the percentage of senescent cells in a culture. Additionally, we utilize immunofluorescence staining to detect other senescence-associated markers, such as p16INK4a and p21CIP1, which are involved in cell cycle regulation and senescence-associated secretory phenotype (SASP).
• Furthermore, we employ gene expression analysis techniques, such as quantitative real-time polymerase chain reaction (qRT-PCR), to assess changes in the expression levels of genes associated with cellular senescence. These approaches enable us to gain a comprehensive understanding of the molecular and cellular changes that occur during the aging process in epithelial cells.

At CD BioSciences, our team of scientists possesses extensive knowledge and expertise in studying cellular senescence and epithelial cell biology. We leverage advanced technologies and laboratory equipment to ensure accurate and reliable results. If you are interested in our services, please feel free to contact us or make an online inquiry.

References

1. Giri S, et al. An in vitro senescence model of gingival epithelial cell induced by hydrogen peroxide treatment. Odontology, 2022, 110 (1): 44-53.
2. Biraud M, et al. Doxorubicin induces senescence in intestinal epithelial cells. bioRxiv, 2021, 428764.