Custom Models for Preclinical Aging Research

Our company provides custom in vitro and in vivo models of skin aging by using UV radiation, oxidative stress induction, and long-term culture of aged skin equivalents. We offer flexible experimental designs to simulate photoaging, chronological aging, and stress-induced aging, supporting research on anti-aging ingredients, skincare formulations, and therapeutic interventions.

• Human fibroblast aging models
• Keratinocyte aging models
• Bioengineered 3D skin aging models
• Mouse model of skin aging induced by D-galactose
• Mouse models of skin photoaging using UV irradiation

CD BioSciences specializes in innovative research services for the evaluation of skin aging, offering comprehensive biochemical assays and advanced imaging techniques.

• Analysis of skin cell renewal
• Analysis of extracellular matrix synthesis and degradation
• Analysis of senescence marker expression levels, including mitochondrial markers, epidermal markers, markers of the dermo-epidermal junction, and dermal markers
• Evaluation of akin aging through confocal microscopy, dermoscopy, optical coherence tomography

CD BioSciences offers custom in vitro and in vivo cardiac aging models that capture the physiological and pathological features of cardiac aging. We perform high-throughput screening using in vitro models to evaluate large compound libraries and identify promising drug candidates. Our in vivo models enable evaluation of anti-aging drug efficacy while facilitating investigation into the underlying molecular mechanisms and pathways affected by therapeutic interventions.

• Senescent cardiomyocytes
• Senescent cardiac fibroblasts
• Mouse models of cardiac aging
• Drosophila models of cardiac aging

At CD BioSciences, we use advanced imaging techniques to visualize cellular changes. We offer senescence-associated β-galactosidase (SA-β-gal) staining for both cell cultures and cardiac tissue cryosections. Furthermore, we use flow cytometry to characterize specific markers of senescence, such as p16^INK4a and p21^CIP1.

• Measurement of left ventricular dimensions
• Analysis of echocardiographic systolic function
• Analysis of echocardiographic diastolic function
• Analysis of myocardial performance index (MPI)

Liver aging models are developed using primary human hepatocytes, hepatic stellate cells (HSCs), and rodent models to replicate the structural and functional alterations that occur during hepatic aging. We induce senescence through various methods, including exposure to senescence-inducing agents (e.g., D-galactose, oxidative stress) or genetic manipulation. Our models allow clients to assess the effects of age-related changes on cellular function, gene expression, and responses to therapeutic agents.

• In vitro models of hepatic senescence
• Animal models of liver aging

CD BioSciences specializes in customizing lung aging models that facilitate comprehensive research and drug discovery in this vital area. Our expertise encompasses a range of in vitro and in vivo models, customized to address specific research objectives

• Models of the aged lung epithelium
• Pulmonary fibrosis murine models with natural aging
• Senescence-accelerated mouse (SAM) models of the aging lung

CD BioSciences helps clients customize aging kidney models using various species and techniques. Our models provide a platform for studying the underlying mechanisms of renal aging, assessing drug efficacy, and exploring potential anti-aging therapeutics.

• Aging models of renal tubular epithelial cells
• Rat models of aging kidney
• Klotho knockout (KO) mice
• Canine models of aging kidney

We provide integrated services for the analysis of cellular senescence, structural changes, and functional decline in the aging kidney, helping clients elucidate mechanisms of renal aging and evaluate potential anti-aging therapeutics.

• Analysis of glomerular aging
• Analysis of the structural integrity of nephron segments
• Analysis of age-related decline in GFR
• Analysis of tubular function

CD BioSciences employs various techniques to develop custom aging skeletal muscle models, catering to diverse research needs. Our expertise encompasses a range of in vitro and in vivo models. Our models of muscle aging enable clients to investigate the underlying mechanisms of muscle aging, screen potential therapeutics, and accelerate the development of innovative interventions.

• In vitro models of aging skeletal muscle
• Rodent models for skeletal muscle senescence
• Zebrafish models for skeletal muscle senescence