Telomere Length Test for Mouse Liver Tissue

The telomere length test for mouse liver tissue measures the size of telomeres, which are protective caps at the end of chromosomes in your cells. Telomeres shorten over time, so telomere length can be used as a marker of biological aging. This test can be used to measure the health of mouse liver tissue and to assess the effects of age-related diseases and treatments.

CD BioSciences can provide telomere length measurements for mouse liver tissue. This can help researchers understand the state of cellular health and aging and the effects of various treatments and interventions on cells.

Telomere Length in Mouse Liver Tissue

The liver is a complex metabolic organ. There is growing evidence that it undergoes all the cellular features of aging. This can increase the risk of developing liver and systemic diseases. Most of the current studies on aging and telomeres have focused on leukocyte telomere length. However, in a study of the effects of aging on telomeres in different tissues of mice, telomere length was found to shorten in the liver up to 25 months of age.

Our Services

Our service is focused on measuring telomere length using the Telomere Length Aging Clock platform. We provide our clients with the required telomere length data from mouse liver tissue samples to evaluate the state of cellular health and aging.

Fig. 1 Service flow for telomere length measurement.

Our specific procedure for measuring telomere length in mouse liver tissue involves extracting DNA from the tissue, quantitative PCR, and calculating the ratio of telomere length to the genome. First, we will isolate DNA samples from mouse liver tissue. Then, we will use qPCR to amplify and measure the repeat length of telomeric DNA. Finally, we will use telomeric DNA repeat length to calculate the telomere length to genome ratio.

It is worth noting that not only did we use predominantly hepatocellular liver tissue for many of our studies on aging livers. We also offer telomere testing services for three other hepatocyte types, including,

• Hepatocytes
• Liver endothelial sinusoidal cells
• Hepatic stellate cells
• Küpffer cells

Sample Requirements

You will need to follow the sample requirements below to submit your sample to be tested. For mouse liver tissue samples to be suitable for telomere length testing, they should be freshly collected, snap-frozen in liquid nitrogen, and kept at -80°C until further processing.

• Mouse liver tissues should be free of contamination.
• The sample size should be between 50-100 mg.
• Further processing should be done within 24 hours of collection.
• If you need the remaining samples after the test is completed, we will return the samples.

We specialize in evaluating telomere length in a variety of samples, and telomere length analysis is designed for use by researchers. Our results are easy to understand and focus more on the aging effects of telomeres.

For those scientists interested in learning more about how telomeres affect health and disease, please contact us for more of our highly recommended products and services.

Reference

1. Hunt N J, et al. Hallmarks of aging in the liver. Computational and structural biotechnology journal, 2019, 17, 1151-1161.

Our services are for research use only and not for any clinical use.