Numerous studies have shown the oxidative stress status of the individual affects the telomere status, which telomere length can be regulated through multiple mechanisms including oxidative stress. Therefore, the use of antioxidants may have the effect of slowing down the rate of telomere shortening and thus slowing down aging.

The effect of antioxidants on telomere length can be evaluated through in vitro study protocols. CD BioSciences provides services to study the effect of antioxidants on telomere length according to rigorous protocols, and will provide detailed analysis reports for clients' reference.

Oxidative Stress Affects Telomere Status

Oxidative stress damage accelerates telomere shortening and drives the aging process. Some external factors such as environment, behavior, diet, and self-induced factors such as inflammation can produce oxidative stress damage, which in turn interferes with telomere maintenance mechanisms and leads to telomere instability.

Fig 1. Effect of oxidative stress on telomere length in aging (Gavia-García G, et al. 2021)

Therefore, some investigators suggest that antioxidants may have a delaying effect on telomere shortening during aging. They can reduce oxidative stress damage to affect telomere length regulation.

Our Cell Experiments In Vitro

We use multiple in vitro models of human cells to study the effects of antioxidants on telomere length status under normal culture conditions and under oxidative stress conditions. The entire cell flow protocol is provided, including all consumables used.

• Experimental materials. We provide the necessary materials for cellular experiments, including culture media, buffered salt solutions, and essential growth substances, which can be configured according to the specific cell line of choice.
• Cell culture. The cells are cultured in standard culture conditions in essential medium. Normal and oxidative stress environments are cultured in parallel. The final concentration of antioxidants used is determined by gradient concentration experiments and applied equally to both experimental groups.
• Cell assay. Cellular assay service options include cell growth and viability assays, DNA extraction, telomerase activity assays, telomere length measurements, and more.

Technical Route

In vitro studies, we investigate the effect of antioxidants on telomere length under normal environmental and oxidative stress conditions. We mainly use a cellular model of senescence development, induce oxidative stress, and add antioxidant protector compounds to simulate and validate their effects.

What Results We Provide

• Report on the treatment of oxidative stress injury by antioxidants. Cellular models allow in vitro validation and analysis of the effects of specific antioxidants on cell morphology, characteristics, and oxidative stress damage.
• Report on the effect of antioxidants on telomere length. Not only can we compare inter- and intra-cellular differences in telomere length, but we can also assess the prior association of all our study variables with telomere length.

We offer a wider variety and more stable in vitro study protocols to design telomere length impact studies. With experienced professionals and advanced technology platforms, CD BioSciences is committed to providing the highest level of telomere research services. For more services, please contact us.

References

1. Gavia-García G, et al. Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging. Biology, 2021, 10(4):253.
2. Badás E P, et al. Ageing and reproduction: antioxidant supplementation alleviates telomere loss in wild birds. Journal of Evolutionary Biology, 2015, 28(4): 896-905.

Our services are for research use only and not for any clinical use.